Sry-negative XX sex reversal in the American cocker spaniel dog.
Sry-negative XX sex reversal in the American cocker spaniel dog.
复制标题
美国可卡犬的 Sry 阴性 XX 性逆转。
DOI：
10.1002/mrd.1080410304
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Hershfield,B
中科院分区：
文献类型：
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作者：
Meyers-Wallen,VN;Palmer,VL;Acland,GM;Hershfield,B
Sry基因产物在通过睾丸诱导的男性性别决定中起着重要作用。然而，在SRY阴性的XX性别逆转的人类中也有睾丸发育的报道。美国可卡犬的XX性别逆转，作为一种常染色体隐性遗传特征，可能是这种疾病的同源物。本研究的目的是确定受感染犬的基因组DNA中是否存在Sry高流动性组（HMG）盒。保守的Sry HMG box和次黄嘌呤磷酸核糖基转移酶（HPRT）序列作为聚合酶链反应的引物。从正常雄性犬的基因组DNA中克隆出一个167 bp的犬Y特异性Sry HMG盒序列。内部引物在正常雄性犬中产生了104 bp的Sry HMG盒产物，而在雌性犬或XX性别反转犬中则没有。平行反应产生所有狗的HPRT产物。结果表明，XX性反转犬基因组DNA中不存在Sry HMG盒。我们推测，在该模型中，在缺乏Sry的情况下，睾丸分化级联的激活是由于常染色体基因突变。©1995 Wiley‐Liss, Inc。
The Sry gene product serves an important function in male sex determination through testis induction. However, testicular development has been reported in SRY‐negative XX sex reversed humans. XX sex reversal of the American cocker spaniel, inherited as an autosomal recessive trait, may be a homolog of this disorder. The purpose of this study was to determine whether the Sry high mobility group (HMG) box is present in genomic DNA of affected dogs. Conserved Sry HMG box and hypoxanthine phosphoribosyltransferase (HPRT) sequences were used as primers in polymerase chain reactions. A 167 bp Y‐specific canine Sry HMG box sequence was cloned from genomic DNA of normal male dogs. Internal primers generated a 104 bp Sry HMG box product from normal males, but not from females or XX sex reversed dogs. Parallel reactions generated an HPRT product from all dogs. Results indicate that the Sry HMG box is absent in genomic DNA of XX sex reversed dogs. We speculate that activation of the testis differentiation cascade in the absence of Sry in this model is due to a mutant autosomal gene. © 1995 Wiley‐Liss, Inc.