Doublesex gene expression in the central nervous system of Drosophila melanogaster
Doublesex gene expression in the central nervous system of Drosophila melanogaster
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DOI：
10.1080/01677060216292
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发表时间：
2002-10-01
影响因子：
1.9
通讯作者：
Park, JH
中科院分区：
文献类型：
--
作者：
Lee, G;Hall, JC;Park, JH
尽管一些行为遗传学研究已经表明doublex（dsx）在神经组织中发挥的作用，但尚未证明该基因的产物实际上存在于中枢神经系统（CNS）中。在这份报告中，我们描述了细胞，空间和时间的dsx基因产物的表达模式，在发展中国家和成人中枢神经系统应用RT-PCR和免疫组化程序。dsx基因产物在3龄幼虫、蛹和成虫的中枢神经系统中检测到。DSX免疫反应性信号在大脑内和腹神经索的胸腹神经节中观察到。大多数，但不是全部，细胞推断含有DSX蛋白（通过抗体特异性的遗传控制的结果）被进一步确定为神经元（通过共表达标记这种CNS细胞类型的蛋白质）。DSX随时间变化的表达最显着地观察到在迅速变态的早期和中期蛹阶段，这表明该基因有助于建立性二态性神经元结构，这使成年人的性行为。DSX免疫反应性的空间和时间模式的元素也意味着，在成年中枢神经系统内的某些神经元簇中的性二态DSX表达可以参与成熟神经系统的正在进行的操作相对于受DSX突变的求爱行为。
Despite several behavior-genetic studies that have suggested roles played by doublesex (dsx) in neural tissues, it has not been demonstrated that the products of this gene are actually present in the central nervous system (CNS). In this report, we describe the cellular, spatial, and temporal expression patterns of dsx gene products in the developing and adult CNS by applying RT-PCR and immunohistochemical procedures. dsx gene products were detected in the CNS of 3rd-instar larvae, pupae, and adults. DSX-immunoreactive signals were observed within the brain and in both the thoracic plus abdominal ganglia of the ventral nerve cord. Most, but not all, cells inferred to contain DSX proteins (by the results of genetic controls for antibody specificity) were further determined to be neurons (by coexpression of a protein that marks such CNS cell types). Temporally varying expression of DSX was most prominently observed in the rapidly metamorphosing early and mid-pupal stages, suggesting that this gene contributes to establishment of sexually dimorphic neuronal structures which subserve adult sexual behaviors. Elements of the spatial and temporal patterns of DSX immunoreactivity also imply that sexually dimorphic dsx expression in certain neuronal clusters within the adult CNS could participate in ongoing operations of the mature nervous system with respect to the courtship behaviors that are affected by dsx mutations.