Characterization of crystals of an intact monoclonal antibody for canine lymphoma.
Characterization of crystals of an intact monoclonal antibody for canine lymphoma.
复制标题
犬淋巴瘤完整单克隆抗体晶体的表征。
DOI：
10.1016/0022-2836(91)90731-k
复制
发表时间：
1991
影响因子：
5.6
通讯作者：
McPherson,A
中科院分区：
文献类型：
--
作者：
Larson,S;Day,J;Greenwood,A;Skaletsky,E;McPherson,A
用聚乙二醇8000蒸气扩散法制备了针对犬淋巴瘤细胞的IgG2a亚类单抗。这些晶体的边缘偶尔会有近一毫米的长度，已经通过X射线衍射进行了检查。晶体属三斜空间群，晶胞参数为a=66·39 Å，b=77·34 Å，c=101·42 Å，α=87·60°，β=92·55°，γ=97·54°，晶胞体积V=4·84×10 5 Å3。三维X射线衍射数据被收集到2.8？分辨率，自旋函数计算显示一个明显的峰值，至少表示一个近似的非晶体二分轴。
A monoclonal antibody of the subclass IgG2a specific for canine lymphoma cells has been crystallized by vapor diffusion from polyethylene glycol 8000. The crystals, which occasionally measure nearly a millimeter on edge, have been examined by X-ray diffraction. The crystals are of triclinic space group P1 with unit cell parameters of a= 66· 39 A ̊, b= 77· 34 A ̊, c= 101· 42 A ̊, α= 87· 60°, β= 92· 55°, γ= 97· 54° and cell volume of V= 4· 84× 10 5 A ̊ 3. There is one entire antibody molecule as the asymmetric unit of the crystals. Three-dimensional X-ray diffraction data have been collected to 2· 8 Å resolution and a self rotation function calculation shows a pronounced peak indicating at least an approximate non-crystallographic dyad axis.