HIV-1 Tat and cocaine coexposure impacts piRNAs to affect astrocyte energy metabolism
HIV-1 Tat and cocaine coexposure impacts piRNAs to affect astrocyte energy metabolism
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DOI：
10.2217/epi-2021-0252
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发表时间：
2022-02-16
期刊：
影响因子：
3.8
通讯作者：
Samikkannu, Thangavel
中科院分区：
文献类型：
--
作者：
Doke, Mayur;Kashanchi, Fatah;Samikkannu, Thangavel
目的：了解HIV感染和可卡因暴露对人原代星形胶质细胞皮尔纳表达的影响。材料和方法：我们使用小RNA测序分析来研究HIV-1达特和可卡因共暴露对人原代星形胶质细胞中piRNA表达的影响。结果：我们鉴定了27，700个piRNA，并通过小RNA下一代测序对其进行了分析。HIV-1达特和可卡因共暴露显著改变了总共239个piRNA。我们还鉴定了PIWIL 1、PIWIL 2、PIWIL 3和PIWIL 4作为受可卡因和HIV-1达特共同暴露影响的piRNA的相互作用伴侣。这些皮尔纳靶标的表达水平的表观遗传变化与能量代谢和神经变性的京都基因和基因组百科全书途径相关。结论：这些发现提供了可卡因暴露和HIV感染影响皮尔纳、PIWIL 1、PIWIL 2、PIWIL 3和PIWIL 4的表达水平的证据。
Aim: To understand the effect of HIV infection and cocaine exposure on piRNA expression in human primary astrocytes. Materials & methods: We used small RNA sequencing analysis to investigate the impacts of HIV-1 Tat and cocaine coexposure on the expression of piRNAs in human primary astrocytes. Results: We identified 27,700 piRNAs and analyzed them by small RNA next-generation sequencing. A total of 239 piRNAs were significantly altered by HIV-1 Tat and cocaine coexposure. We also identified PIWIL1, PIWIL2, PIWIL3 and PIWIL4 as interacting partners of piRNAs that were affected by cocaine and HIV-1 Tat coexposure. Epigenetic changes in the expression levels of these piRNA targets were associated with Kyoto Encyclopedia of Genes and Genomes pathways of energy metabolism and neurodegeneration. Conclusion: These findings provide evidence that cocaine exposure and HIV infection affect the expression levels of piRNA, PIWIL1, PIWIL2, PIWIL3 and PIWIL4.