Molybdenum and iron mutually impact their homeostasis in cucumber (Cucumis sativus) plants.
Molybdenum and iron mutually impact their homeostasis in cucumber (Cucumis sativus) plants.
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DOI：
10.1111/nph.14214
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发表时间：
2017-02
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影响因子：
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通讯作者：
G. Vigani;D. Di Silvestre;A. Agresta;S. Donnini;P. Mauri;C. Gehl;F. Bittner;I. Murgia
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作者：
G. Vigani;D. Di Silvestre;A. Agresta;S. Donnini;P. Mauri;C. Gehl;F. Bittner;I. Murgia
钼（Mo）和铁（Fe）是植物代谢中关键酶活性所必需的微量营养元素。在这里，我们调查了黄瓜（黄瓜）中存在的相互控制的钼和铁的动态平衡。植物生长在单一或组合钼和铁饥饿。测定生理参数，分析组织离子组和根线粒体的离子组和蛋白质组，并评价钼辅因子（Moco）的合成和脱氢酶的活性。铁和钼被发现影响对方的总吸收和分布在组织内和线粒体水平，与铁营养状况占主导地位的钼稳态和影响钼的可用性的形式Moco的β-淀粉酶。铁饥饿引发莫科生物合成和影响的酶，其主要影响硝酸还原酶和黄嘌呤脱氢酶，都参与氮同化和动员，并对线粒体偕胺肟还原组分。这些结果，以及在根线粒体中差异表达的> 100种蛋白质的鉴定，突出了线粒体在Fe和Mo稳态协调中的核心作用，并使我们能够提出连接Mo和Fe稳态的分子相互作用的第一个模型。
Molybdenum (Mo) and iron (Fe) are essential micronutrients required for crucial enzyme activities in plant metabolism. Here we investigated the existence of a mutual control of Mo and Fe homeostasis in cucumber (Cucumis sativus). Plants were grown under single or combined Mo and Fe starvation. Physiological parameters were measured, the ionomes of tissues and the ionomes and proteomes of root mitochondria were profiled, and the activities of molybdo-enzymes and the synthesis of molybdenum cofactor (Moco) were evaluated. Fe and Mo were found to affect each other's total uptake and distribution within tissues and at the mitochondrial level, with Fe nutritional status dominating over Mo homeostasis and affecting Mo availability for molybdo-enzymes in the form of Moco. Fe starvation triggered Moco biosynthesis and affected the molybdo-enzymes, with its main impact on nitrate reductase and xanthine dehydrogenase, both being involved in nitrogen assimilation and mobilization, and on the mitochondrial amidoxime reducing component. These results, together with the identification of > 100 proteins differentially expressed in root mitochondria, highlight the central role of mitochondria in the coordination of Fe and Mo homeostasis and allow us to propose the first model of the molecular interactions connecting Mo and Fe homeostasis.