From Whole-body Sections Down to Cellular Level, Multiscale Imaging of Phospholipids by MALDI Mass Spectrometry
From Whole-body Sections Down to Cellular Level, Multiscale Imaging of Phospholipids by MALDI Mass Spectrometry
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DOI：
10.1074/mcp.o110.004259
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发表时间：
2011-02-01
影响因子：
7
通讯作者：
Caprioli, Richard M.
中科院分区：
文献类型：
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作者：
Chaurand, Pierre;Cornett, Dale S.;Caprioli, Richard M.
在仪器和样品制备方法方面的重大进展最近扩大了MALDI成像质谱仪分析组织样品中天然存在的磷脂和其他内源性代谢物的潜力。在这里，我们探讨了用MALDI飞行时间质谱仪从不同维度的薄组织切片中成功分析和成像磷脂所必需的一些要求。我们讨论与全身切片的成像相关的方法学问题，例如从模型实验室动物切割的切片，通常从单个器官制备的中等尺寸的切片，以及从这些切片以细胞尺度空间分辨率成像感兴趣区域的要求。我们还回顾了MALDI成像MS技术在化合物鉴定方面存在的局限性。最后，我们对与数据开发和管理相关的重要问题进行了展望，这些问题需要得到解决，以最大限度地了解所研究的组织标本的生物学意义。分子与细胞蛋白质组学10：10.1074/mcp.O110.004259，1-11,2011年。
Significant progress in instrumentation and sample preparation approaches have recently expanded the potential of MALDI imaging mass spectrometry to the analysis of phospholipids and other endogenous metabolites naturally occurring in tissue specimens. Here we explore some of the requirements necessary for the successful analysis and imaging of phospholipids from thin tissue sections of various dimensions by MALDI time-of-flight mass spectrometry. We address methodology issues relative to the imaging of whole-body sections such as those cut from model laboratory animals, sections of intermediate dimensions typically prepared from individual organs, as well as the requirements for imaging areas of interests from these sections at a cellular scale spatial resolution. We also review existing limitations of MALDI imaging MS technology relative to compound identification. Finally, we conclude with a perspective on important issues relative to data exploitation and management that need to be solved to maximize biological understanding of the tissue specimen investigated. Molecular & Cellular Proteomics 10:10.1074/mcp.O110.004259, 1-11, 2011.