PROTECTIVE IMMUNITY AGAINST BRUGIA-MALAYI INFECTIVE LARVAE IN MICE .1. PARAMETERS OF ACTIVE AND PASSIVE-IMMUNITY
PROTECTIVE IMMUNITY AGAINST BRUGIA-MALAYI INFECTIVE LARVAE IN MICE .1. PARAMETERS OF ACTIVE AND PASSIVE-IMMUNITY
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DOI：
10.4269/ajtmh.1989.41.650
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发表时间：
1989-12-01
影响因子：
3.3
通讯作者：
TANAKA, H
中科院分区：
文献类型：
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作者：
HAYASHI, Y;NAKAGAKI, K;TANAKA, H
用不同来源的抗原在不同品系的小鼠中研究了对马来丝虫感染性幼虫的保护性免疫。以下品系的小鼠在初次腹腔内攻毒后2周内对感染性幼虫发育敏感：BALB/c、C3 H/HeJ、C3 H/NeN、C3 H/HeJms、C57 BL/6 Jms和DDD。与沙鼠相比，BALB/c小鼠经辐射减毒或灭活微丝蚴免疫后，对感染性幼虫的抵抗力更强。然而，杀死的MF未能增强C3 H/HeJ小鼠的抵抗力，尽管C3 H/HeN小鼠受到强烈保护，C3 H/HeJms小鼠受到该抗原的保护程度较低。用磷酸盐缓冲液和十二烷基硫酸钠提取的MF均诱导BALB/c小鼠产生高水平的抵抗。从BALB/c小鼠免疫减毒感染性幼虫幼稚小鼠的抗性转移完成在高水平的保护尼龙羊毛非贴壁脾细胞（T细胞），但不与抗Thy 1.2血清和补体处理的贴壁细胞。相比之下，来自免疫小鼠的血清的保护性要低得多。
Protective immunity against infective larvae of Brugia malayi was studied in different strains of mice using various sources of antigens. The following strains of mice were susceptible to infective larvae development for 2 weeks after primary ip challenge: BALB/c, C3H/HeJ, C3H/NeN, C3H/HeJms, C57BL/6Jms, and DDD. In comparison to gerbils, BALB/c mice developed stronger resistance to infective larvae after immunization with irradiation attenuated larvae or with killed microfilariae (mf). However, killed mf failed to enhance resistance in C3H/HeJ mice, although C3H/HeN mice were strongly protected and C3H/HeJms mice were protected to a lesser degree by this antigen. Extracts of mf with phosphate buffered saline and sodium dodecyl sulfate both induced high levels of resistance in BALB/c mice. Transfer of resistance from BALB/c mice immunized with attenuated infective larvae to naive mice was accomplished at a high level at protection with nylon wool nonadherent spleen cells (T cells) but not with adherent cells treated with anti-Thy 1.2 serum and complement. In contrast, sera from immunized mice were much less protective.