Transcriptome Analysis Reveals the Accumulation Mechanism of Starch in Chinese Yam (Dioscorea opposite Thunb.) Tubers Using RNA-Seq and Iso-Seq
Transcriptome Analysis Reveals the Accumulation Mechanism of Starch in Chinese Yam (Dioscorea opposite Thunb.) Tubers Using RNA-Seq and Iso-Seq
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DOI：
10.1166/sam.2022.4205
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发表时间：
2022-02-01
影响因子：
0.9
通讯作者：
Huo, Xiuwen
中科院分区：
文献类型：
--
作者：
Gao, Yuanli;Zhang, Yanfang;Huo, Xiuwen
山药块茎的淀粉组成影响着山药的品质和产量，深入研究山药淀粉积累的机理具有重要意义。本研究以淀粉含量差异显著的两个山药品种毕克奇（学名：Arabrea opposita Bikeqi）和大河长芋（学名：Arabrea opposita Dahechangyu）为试材。使用RNA-seq和Iso-seq对来自7个发育阶段的块茎的转录组进行测序。筛选出与淀粉和蔗糖代谢密切相关的差异基因，并对其功能进行了分析。结果表明，两个山药品种的淀粉含量分别在150和165 d时达到最大值，150 d时差异最大。共注释了21623个单一基因，通过比较各发育阶段与块茎膨大开始（90天）的差异，鉴定出8378个品种内差异基因。在同一时期的两个品种中共鉴定出5177个差异基因。GO和KEGG用于IP：14.98.160.66 On：Fri，13 May 2022 09：20：14分析这些差异基因，筛选出多种与淀粉和蔗糖代谢密切相关的关键基因。这些结果有助于我们更好地理解山药淀粉积累的机理。
The starch composition of tubers affects the quality and yield of yam, and it is of great significance to deeply investigate the mechanism of starch accumulation in yam. In this study, two yam varieties with significant differences in starch content, Bikeqi (Dioscorea opposita Bikeqi) and Dahechangyv (Dioscorea opposita Dahechangyu), were used as materials. The transcriptome of tubers from seven developmental stages were sequenced using RNA-seq and Iso-seq. Differential genes closely contacted with the metabolism of starch and sucrose were screened and the functions were analysed. The results showed that, the starch content of the two yam varieties reached the maximum at 150 and 165 days respectively, and their difference was the largest at 150 days. Total 21623 unigenes were annotated, and 8378 differential genes were identified within varieties by comparing each developmental stage with the beginning of tuber expansion (90 days), respectively. A total of 5177 differential genes were identified in two varieties in the same period. GO and KEGG were used to IP: 14.98.160.66 On: Fri, 13 May 2022 09:20:14 analyze these differential genes, a variety of key genes closely related to starch and sucrose metabolism were screened. These results help us to better understand the mechanism of starch accumulation in yam.