Genetic markers for oncogenes, growth factors, and cystic fibrosis.
Genetic markers for oncogenes, growth factors, and cystic fibrosis.
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DOI：
10.1007/978-3-642-74621-5_63
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
M. Dean;C. Stewart;A. Perry;B. Gerrard;T. Beck;U. Rapp;M. Drumm;M. Iannuzzi;F. Collins;S. O’Brien
中科院分区：
文献类型：
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作者：
M. Dean;C. Stewart;A. Perry;B. Gerrard;T. Beck;U. Rapp;M. Drumm;M. Iannuzzi;F. Collins;S. O’Brien
分子生物学技术对人类遗传学的进步产生了巨大的影响。特别是，限制性片段长度多态性（RFLPs）的发展使研究人员能够为人类基因组的几乎任何区域生成遗传标记。大多数RFLPs发生在突变产生或删除限制性内切酶的识别位点时，产生大小改变的DNA片段。在最简单的情况下，这将产生两个等位基因。与该片段杂交的DNA探针将在来自不同个体的DNA中检测到这些等位基因的存在。用于检测RFLPs的探针来自克隆基因和随机分离的DNA片段。因此，每个RFLP都是染色体上精确位置的遗传标记。
The techniques of molecular biology have had a dramatic effect on the advancement of human genetics. In particular, the development of restriction fragment length polymorphisms (RFLPs) has allowed researchers to generate genetic markers for virtually any region of the human genome. Most RFLPs occur when a mutation creates or deletes a recognition site for a restriction enzyme, generating a DNA fragment of altered size. In the simplest case this will create two alleles. A DNA probe which hybridizes to this fragment will detect the presence of these alleles in the DNA from different individuals. Probes used to detected RFLPs have been derived from both cloned genes and randomly isolated DNA segments. Thus, each RFLP is a genetically inherited marker for a precise location on a chromosome.