Human white adipocytes express the cold receptor TRPM8 which activation induces UCP1 expression, mitochondrial activation and heat production
Human white adipocytes express the cold receptor TRPM8 which activation induces UCP1 expression, mitochondrial activation and heat production
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DOI：
10.1016/j.mce.2013.12.005
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发表时间：
2014-03-05
影响因子：
4.1
通讯作者：
Vettor, Roberto
中科院分区：
文献类型：
--
作者：
Rossato, Marco;Granzotto, Marnie;Vettor, Roberto
哺乳动物拥有两种类型的脂肪组织，白色（WAT）和棕色（BAT）。解偶联蛋白I（UCP1）是BAT的标志，是冷诱导产热的关键参与者。在本研究中，我们证明了人白色脂肪细胞表达冷感受受体TRPM 8，薄荷醇和icilin激活该受体可诱导[Ca 2 +]（i）和UCP 1表达增加，线粒体膜电位增加，葡萄糖摄取和产热增加。TRPM 8活化后人白色脂肪细胞中“棕色样”表型的诱导得到线粒体形态及其细胞内定位的超微结构形态学变化的支持。总之，人白色脂肪细胞表达冷受体TRPM 8，其激活诱导其“布朗宁”。支持该受体在控制脂肪组织代谢和身体能量平衡中的可能作用。(C)2013爱思唯尔爱尔兰有限公司版权所有。
Mammals possess two types of adipose tissue, white (WAT) and brown (BAT). The uncoupling protein I (UCP1) is a hallmark of BAT, being the pivotal player for cold-induced thermogenesis. WAT can acquire BAT characteristics with up-regulation of UCP1 after cold exposure or adrenergic stimulation.In the present study we demonstrated that human white adipocytes express the cold-sensing receptor TRPM8 which activation by menthol and icilin induced a rise in [Ca2+](i) and UCP1 expression, increased mitochondrial membrane potential, glucose uptake and heat production. The induction of "brown-like" phenotype in human white adipocytes after TRPM8 activation was supported by ultrastructural morphological changes of mitochondria] morphology and of their intracellular localization, with no modifications of the genes regulating mitochondrial biogenesis.In conclusion human white adipocytes express the cold receptor TRPM8 which activation induces their "browning" supporting a possible role of this receptor in the control of adipose tissue metabolism and body energy balance. (C) 2013 Elsevier Ireland Ltd. All rights reserved.