Proteomic investigation of changes in human vastus lateralis muscle in response to interval-exercise training
Proteomic investigation of changes in human vastus lateralis muscle in response to interval-exercise training
复制标题
DOI：
10.1002/pmic.200900068
复制
发表时间：
2009-11-01
期刊：
影响因子：
3.4
通讯作者：
Burniston, Jatin G.
中科院分区：
文献类型：
--
作者：
Holloway, Kathryn V.;O'Gorman, Martin;Burniston, Jatin G.
以前没有研究使用蛋白质组学来研究运动训练对人体骨骼肌的影响。5名娱乐活跃的男性完成了一项为期6周的训练计划，包括每周3次，利用6次最大摄氧量（VO（2）max）下的1分钟比赛，其中4分钟为50%VO（2）max。在训练干预前后的标准时间对股外侧肌进行活检。蛋白质表达谱分析采用2-DE凝胶的差异分析进行，并辅以LC-MALDI MS/MS对1-DE凝胶泳道片段的胰蛋白酶肽进行定量分析（iTRAQ）。
No previous study has used proteomics to investigate the effects of exercise training on human skeletal muscle. Five recreationally active men completed a 6-wk training programme involving three sessions per week, utilising six 1-min bouts at maximum oxygen uptake (V O(2)max) interspersed with 4 min at 50% V O(2)max. Vastus lateralis was biopsied at standardised times before and after the training intervention. Protein expression profiling was performed using differential analysis of 2-DE gels; complemented with quantitative analysis (iTRAQ) of tryptic peptides from 1-DE gel lane-segments using LC-MALDI MS/MS. Interval training increased average V O(2)max (7%; p