Juvenile Dungeness crabs ( Metacarcinus magister ) selectively integrate and modify the fatty acids of their experimental diets
Juvenile Dungeness crabs ( Metacarcinus magister ) selectively integrate and modify the fatty acids of their experimental diets
复制标题
幼年珍宝蟹（Metacarcinus magister）选择性地整合并改变其实验饮食中的脂肪酸
DOI：
10.1098/rstb.2020.0038
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Galloway, Aaron W.
中科院分区：
文献类型：
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作者：
Thomas, Michael D.;Schram, Julie B.;Clark-Henry, Zade F.;Yednock, Bree K.;Shanks, Alan L.;Galloway, Aaron W.
珍宝蟹（Metacarcinus magister）在东北太平洋沿海地区具有重要的生态和经济意义，但目前人们对它们在野外的摄食行为或自然饮食知之甚少。营养生物标志物，例如脂肪酸 (FA)，可用于揭示营养相互作用。我们使用两个喂养实验来评估喂养不同已知食物的幼蟹 FA 组成的差异，以评估它们如何修改膳食 FA 并将其整合到自己的组织中，并确定螃蟹 FA 是否反映了六周内的饮食变化。然后将这些实验结果与未确定饮食的野生捕获幼蟹的 FA 特征进行比较。我们发现，幼年珍宝蟹饲喂不同的食物，将膳食 FA 吸收到其组织中，并且在进行多变量统计分析时，其 FA 特征各不相同。实验喂养的幼蟹所含的最丰富的长链多不饱和脂肪酸（LCPUFA，>C20）比其食物中的比例更高。喂食缺乏 LCPUFA，特别是 DHA（22:6ω3，二十二碳六烯酸）的食物的螃蟹，无法比喂食其他食物的螃蟹存活或生长得更慢。这表明 LCPUFA 对于该物种具有重要的生理意义，并表明这些 FA 的生物合成没有发生或不足以满足其需求。本文是主题“脂质作为‘营养生物标志物’的下一个前景：消费者改变膳食脂肪酸的证据和意义”的一部分。
Dungeness crabs (Metacarcinus magister) are ecologically and economically important in the coastal Northeast Pacific, yet relatively little is currently known about their feeding behaviour in the wild or their natural diet. Trophic biomarkers, such as fatty acids (FA), can be used to reveal trophic interactions. We used two feeding experiments to assess differences in FA composition of juvenile crabs fed different known foods to evaluate how they modify and integrate dietary FA into their own tissues and determine whether crab FA reflect diet changes over a six-week period. These experimental results were then compared with the FA signatures of wild caught juvenile crab with undetermined diets. We found that juvenile Dungeness crabs fed different foods assimilated dietary FA into their tissues and were distinct in their FA signatures when analysed with multivariate statistics. Experimentally fed juvenile crabs contained greater proportions of the most abundant long-chain polyunsaturated fatty acids (LCPUFA, >C20) than their foods. Crabs fed foods lacking in LCPUFA, particularly DHA (22:6ω3, docosahexaenoic acid), did not survive or grew slower than crabs fed other foods. This suggests that LCPUFA are physiologically important for this species and indicates biosynthesis of these FA does not occur or is not sufficient to meet their needs.This article is part of the theme issue ‘The next horizons for lipids as ‘trophic biomarkers’: evidence and significance of consumer modification of dietary fatty acids’.