THE DEVELOPMENT OF A CLINICAL PROTOCOL FOR THE RADIOLABELLING OF MIXED LEUKOCYTES WITH TC-99M-HEXAMETHYLPROPYLENEAMINE OXIME
THE DEVELOPMENT OF A CLINICAL PROTOCOL FOR THE RADIOLABELLING OF MIXED LEUKOCYTES WITH TC-99M-HEXAMETHYLPROPYLENEAMINE OXIME
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DOI：
10.1097/00006231-198806000-00010
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发表时间：
1988-06-01
影响因子：
1.5
通讯作者：
CARROLL, MJ
中科院分区：
文献类型：
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作者：
DANPURE, HJ;OSMAN, S;CARROLL, MJ
为设计99 Tcm-六甲基丙烯胺肟（99 Tcm-HMPAO）标记混合白细胞的方法，测定了HMPAO浓度、细胞浓度、血浆浓度和抗凝剂对99 Tcm-HMPAO亲脂性复合物摄取的影响，以及99 Tcm在标记细胞上的稳定性。发现细胞摄取迅速，在20-80 μg ml-1范围内与HMPAO浓度无关，但依赖于细胞和血浆浓度。取85 ml全血，加入1 ml ACD-血浆和4 ml含400 MBq 99 Tcm的99 Tcm-HMPAO，室温孵育10 min，效果最佳，用于90例病人。平均标记效率为55%（±13 SD），其中粒细胞占77%，单核细胞占17%，血小板和红细胞占6%。在血浆中孵育时，标记的混合白细胞释放出9%（± 4%SD）的99 Tcm。这主要发生在单核细胞。
To devise a protocol for the radiolabelling of mixed leucocytes with 99 Tc m-hexamethyl-propyleneamine oxime (99 Tc m-HMPAO), the effect of HMPAO concentration, cell concentration, plasma concentration and anticoagulant on the uptake of the lipophilic complex was measured, together with the stability of the 99 Tc m on the labelled cells. It was found that cell uptake was rapid, independent of HMPAO concentration over the range 20–80 μg ml− 1, but dependent on cell and plasma concentration. Incubation of mixed leucocytes from 85 ml blood with 1 ml ACD-plasma and 4 ml 99 Tc m-HMPAO containing 400 MBq 99 Tc m for 10 min at room temperature gave optimum results and was used in 90 patients. The mean labelling efficiency, which was the amount of added 99 Tc m incorporated by the cells, was 55%(±13 SD), of which 77% were incorporated by the granulocytes, 17% by the mononuclear cells and 6% by the platelets and erythrocytes. During alh incubation in plasma 9%(±4% SD) of the 99 Tc m were released from the labelled mixed leucocytes. This occurred predominantly from the mononuclear cells.