Paper Spray and Extraction Spray Mass Spectrometry for the Direct and Simultaneous Quantification of Eight Drugs of Abuse in Whole Blood
Paper Spray and Extraction Spray Mass Spectrometry for the Direct and Simultaneous Quantification of Eight Drugs of Abuse in Whole Blood
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DOI：
10.1021/ac5016408
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发表时间：
2014-08-05
影响因子：
7.4
通讯作者：
Cooks, R. Graham
中科院分区：
文献类型：
--
作者：
Espy, Ryan D.;Teunissen, Sebastiaan Frans;Cooks, R. Graham
已使用纸喷雾或萃取喷雾质谱法在2分钟内以最少的样品制备进行了血液中8种滥用药物的测定。优化了一种方法，用于从单个血斑中定量苯丙胺、甲基苯丙胺、3，4-亚甲二氧基苯丙胺（MDA）、3，4-亚甲二氧基-N-甲基苯丙胺（MDMA）、3，4-亚甲二氧基-N-乙基苯丙胺（MDEA）、吗啡、可卡因和Delta 9-四氢大麻酚（THC）。使用稳定同位素标记的内标物和串联质谱法实现了1-5%相对标准偏差的样品间变化。所有药物的检测限均低于典型的生理和毒理学水平。纸喷雾和提取喷雾各自使用少于10 μ L的全血。这些方法表现出的潜力，进行快速和高通量的检测，选择性的现场多化合物定量筛选非法药物。
Determination of eight drugs of abuse in blood has been performed using paper spray or extraction spray mass spectrometry in under 2 min with minimal sample preparation. A method has been optimized for quantification of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxy-N-methylamphetamine (MDMA), 3,4-methylenedioxy-N-ethylamphetamine (MDEA), morphine, cocaine, and Delta 9-tetrahydrocannabinol (THC) from a single blood spot. Sample to sample variations of 1-5% relative standard deviation were achieved using stable isotope-labeled internal standards and tandem mass spectrometry. Limits of detection for all drugs were below typical physiological and toxicological levels. Paper spray and extraction spray each used less than 10 mu L of whole blood. These methods exhibit the potential for performing rapid and high-throughput assays for selective on-site multicompound quantitative screening of illicit drugs.