iSeq 2.0: A Modular and Interchangeable Toolkit for Interaction Screening in Yeast.
iSeq 2.0: A Modular and Interchangeable Toolkit for Interaction Screening in Yeast.
复制标题
DOI：
10.1016/j.cels.2019.03.005
复制
发表时间：
2019-04
期刊：
影响因子：
9.3
通讯作者：
Xianan Liu;Zhimin Liu;Adam Dziulko;Fangfei Li;Darach Miller;Robert D Morabito;Danielle Francois;Sasha F. Levy
中科院分区：
文献类型：
--
作者：
Xianan Liu;Zhimin Liu;Adam Dziulko;Fangfei Li;Darach Miller;Robert D Morabito;Danielle Francois;Sasha F. Levy
We developed a flexible toolkit for combinatorial screening inSaccharomyces cerevisiae, which generates large libraries of cells, each uniquely barcoded to mark a combination of DNA elements. This interaction sequencing platform (iSeq 2.0) includes genomic landing pads that assemble combinations through sequential integration of plasmids or yeast mating, 15 barcoded plasmid libraries containing split selectable markers (URA3AI,KanMXAI,HphMXAI, andNatMXAI), and an array of ∼24,000 "double-barcoder" strains that can make existing yeast libraries iSeq compatible. Various DNA elements are compatible with iSeq: DNA introduced on integrating plasmids, engineered genomic modifications, or entire genetic backgrounds. DNA element libraries are modular and interchangeable, and any two libraries can be combined, making iSeq capable of performing many new combinatorial screens by short-read sequencing.
