Molecular characterization of orf virus from sheep and goats in Ethiopia, 2008-2013.
Molecular characterization of orf virus from sheep and goats in Ethiopia, 2008-2013.
复制标题
DOI：
10.1186/s12985-016-0489-3
复制
发表时间：
2016-02-29
期刊：
影响因子：
4.8
通讯作者：
Lamien CE
中科院分区：
文献类型：
--
作者：
Gelaye E;Achenbach JE;Jenberie S;Ayelet G;Belay A;Yami M;Loitsch A;Grabherr R;Diallo A;Lamien CE
羊口疮是由痘病毒科副痘病毒属羊口疮病毒（ORFV）引起的绵羊、山羊和野生有蹄类动物的一种传染病。虽然羊口疮是埃塞俄比亚的地方病，但由于它不是世界动物卫生组织规定的应报告疾病，因此很少受到关注。在这项工作中，我们调查了2008年至2013年期间代表埃塞俄比亚五个不同地理位置的羊口疮疫情，分别发生在Amba Giorgis，Gondar zuria，Adet，Debre zeit和Adami Tulu。对18株代表性分离株进行病毒分离和A32 L和B2 L基因序列分析，证实所采集的动物被ORFV感染。系统发育研究和推导的氨基酸谱的比较分析表明，有两个主要的集群的ORFV分离株负责调查的爆发。此外，这两个基因的分析表明，在其他地方遇到的ORFV的变异性有限。这是第一份关于埃塞俄比亚绵羊和山羊ORFV分离株遗传特征的报告。埃塞俄比亚ORFV分离株的分子特征突出了导致绵羊和山羊中的ORF疾病的两个主要簇的循环。需要使用基于实验室的方法和持续监测埃塞俄比亚ORFV分离株，以更好地了解在该国传播的ORFV的动态，并促进控制措施的实施。本文的在线版本（doi：10.1186/s12985-016-0489-3）包含补充材料，可供授权用户使用。
Orf is a contagious disease of sheep, goats and wild ungulates caused by orf virus (ORFV) a member of the genus Parapoxvirus, Poxviridae family. Although orf is endemic in Ethiopia, little attention has been given so far as it is not a notifiable disease by the World Organization for Animal Health. In this work, we have investigated orf outbreaks representing five different geographical locations of Ethiopia, in Amba Giorgis, Gondar zuria, Adet, Debre zeit and Adami Tulu, between 2008 and 2013. The viral isolation and the sequence analysis of the A32L and the B2L genes of eighteen representative isolates confirmed that sampled animals were infected by ORFVs. The phylogenetic study and the comparative analysis of the deduced amino acid profile suggests that there were two main clusters of ORFV isolates which were responsible for the investigated outbreaks. Additionally the analysis of these two genes showed limited variability to ORFVs encountered elsewhere. This is the first report on the genetic characterization of the ORFV isolates from sheep and goats in Ethiopia. The molecular characterization of Ethiopian ORFV isolates highlighted the circulation of two main clusters causing orf disease in sheep and goats. The use of laboratory based methods and a constant monitoring of Ethiopian ORFV isolates is needed to better understand the dynamic of ORFV circulating in the country and facilitate the implementation of control measures. The online version of this article (doi:10.1186/s12985-016-0489-3) contains supplementary material, which is available to authorized users.