Modeling of segmentation clock mechanism in presomitic mesoderm
Modeling of segmentation clock mechanism in presomitic mesoderm
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DOI：
10.1109/iembs.2009.5333510
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发表时间：
2009-11
期刊：
影响因子：
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通讯作者：
A. Kazama;A. Karashima;Norihiro Katayama;M. Nakao
中科院分区：
文献类型：
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作者：
A. Kazama;A. Karashima;Norihiro Katayama;M. Nakao
体节是通过分割被称为体节前中胚层 (PSM) 的间充质组织，以头到尾的顺序依次生成的。这种分段周期性地发生在 PSM 的前端，并且这种周期性分段被认为是由分子钟调节的。在小鼠 PSM 中，与分割相关的基因每 120 分钟改变一次表达，这种循环表达对于规则的体节分割至关重要。在本研究中，对涉及Wnt和Delta-Notch信号通路的分段时钟的分子机制进行了建模，并通过模拟生物学发现来研究模型结构的现实性。构建细胞时钟模型的一维阵列来模拟 PSM 中基因表达的时空动态。模拟结果表明 PSM 上的 Wnt 梯度参与了所关注的动力学。
Somite is sequentially generated in a head-to-tail order by segmentation of the mesenchymal tissue called presomitic mesoderm (PSM). The segmentation occurs periodically at the anterior end of the PSM, and this periodic segmentation has been suggested to be regulated by a molecular clock. In mouse PSM, the segmentation-related genes change their expression every 120 minutes, and this cyclic expression is essential for regular somite segmentation. In this study, a molecular mechanism of segmentation clock involving Wnt and Delta-Notch signaling pathways is modeled, and reality of the model structure is investigated through simulating biological findings. One dimensional array of the cellular clock models is constructed to simulate spatio-temporal dynamics of the gene-expressions in the PSM. The simulation result suggests that the Wnt gradient across the PSM is involved in the dynamics under concern.