A Halophilic Vibrio Isolated from a Case of Chronic Cholecystitis
A Halophilic Vibrio Isolated from a Case of Chronic Cholecystitis
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从一例慢性胆囊炎中分离出的嗜盐弧菌
DOI：
10.1111/j.1348-0421.1981.tb00132.x
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发表时间：
1981
影响因子：
2.6
通讯作者：
K. Sakamoto
中科院分区：
文献类型：
--
作者：
K. Konishi;T. Yamagishi;K. Sakamoto
我们在一位75岁女性慢性胆囊炎患者的胆汁中发现了一种嗜盐弧菌，并检查了其生化特征。这些微生物是革兰氏阴性的短杆状或逗号状，有一些环状。它们有单极鞭毛，但没有蒴果。菌株在1.0 ~ 4.0% NaCl浓度的蛋白胨水中可以生长，在无NaCl和6.0% NaCl浓度的蛋白胨水中不能生长。这些微生物的特点是葡萄糖发酵、过氧化氢酶、氧化酶和鸟氨酸脱羧酶呈阳性，赖氨酸脱羧酶、精氨酸二水解酶呈阴性，葡萄糖无气体。它们对2,4 -二胺- 6,7 -二异丙基蝶啶（0/129）敏感。这些特征表明分离的菌株应该是一种嗜盐弧菌。然而，沙门氏菌-志贺氏菌（SS）、添加蔗糖和溴甲酚紫（SS - SB）的沙门氏菌、麦康基（MacConkey）或硫代硫酸柠檬酸胆盐（TCBS）琼脂板上没有生长。硝酸还原、西蒙斯柠檬酸琼脂、吲哚、o -硝基酚- β - d -半乳糖苷（ONPG）、运动和内皮素水解均呈阳性。脲酶、明胶酶、Voges - Proskauer、苯丙氨酸脱氨酶和丙二酸盐反应均为阴性。苦杏仁苷、阿拉伯糖、纤维二糖、果糖、半乳糖、葡萄糖、乳糖、麦芽糖、甘露醇、水杨酸、淀粉、蔗糖、海藻糖和木糖均可制酸，但甘露糖醇、糖糖醇、肌醇、甘露糖、糖糖、棉子糖、鼠李糖和山梨醇不能制酸。从这些特征来看，该分离物被认为与副溶血性弧菌、霍乱弧菌、创伤弧菌或其他弧菌不同。可以推测，这种分离物代表了另一种嗜盐弧菌。
We found a halophilic vibrio in B bile from a 75‐year‐old female patient with chronic cholecystitis, and examined its biochemical characteristics. The organisms are gram‐negative short rods or comma shaped, with some ring forms. They have a single polar flagellum, but no capsule. The strains can grow in peptone water with 1.0 to 4.0% NaCl, but not with no NaCl or 6.0% NaCl. The characteristics of the organisms are positive dextrose fermentation, catalase, oxidase, and ornithine decarboxylase, and negative lysine decarboxylase, arginine dihydrolase, and absence of gas from glucose. They are sensitive to 2,4‐diamine‐6,7‐diisopropyl pteridine (0/129). These characteristics indicate that the isolated strain should be a halophilic vibrio. However, no growth on Salmonella‐Shigella (SS), SS with added sucrose and bromcresol purple (SS‐SB), MacConkey's or thiosulfate citrate bile salts (TCBS) agar plates was demonstrated. Nitrate reduction, Simmons' citrate agar, indole, o‐nitrophenol‐β‐d‐galactopyranoside (ONPG), motility and esculin hydrolysis were positive. Urease, gelatinase, Voges‐Proskauer, phenylalanine deaminase and malonate reactions were negative. Acid was produced from amygdalin, arabinose, cellobiose, fructose, galactose, glucose, lactose, maltose, mannitol, salicin, starch, sucrose, trehalose, and xylose, but not from adonitol, dulcitol, inositol, mannose, melibiose, raffinose, rhamnose and sorbitol. From these characteristics the isolate is considered to be not identical with V. parahaemolyticus, V. cholerae, V. vulnificus or other vibrios. It can be presumed that this isolate represents another species of halophilic vibrio.