Morphology, ultrastructure and phylogeny of Cyanothece sp (Cyanobacteriaceae: Cyanophyceae) isolated from the eastern Indian Ocean
Morphology, ultrastructure and phylogeny of Cyanothece sp (Cyanobacteriaceae: Cyanophyceae) isolated from the eastern Indian Ocean
复制标题
东印度洋分离的蓝藻（蓝细菌科：蓝藻科）的形态、超微结构和系统发育
DOI：
10.1007/s13131-018-1297-y
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发表时间：
2018
影响因子：
1.4
通讯作者：
Ding Changling
中科院分区：
文献类型：
--
作者：
Zhang Xiaodong;Yang Shuang;Sun Jun;Wu Chao;Wang Jing;Zhang Guicheng;Ding Changling
从印度洋赤道海域成功分离到一株胶液包埋的单细胞绿藻。经显微观察、超微结构特征和遗传鉴定，证实该菌株与具有重要生态意义的蓝藻物种蓝藻亲缘关系密切。细胞单生，呈椭圆形或杆状。该菌株直径较小，平均为2.5~6.5μm。细胞超微结构简单。类囊体区内可见类囊体排列、顶端和角质化。此外，还观察到具有低电子致密区的各种电子透明颗粒以及蓝藻素或糖原颗粒样细胞器和碳氧体。色素组成以叶绿素、β-胡萝卜素、玉米黄质和一种未知色素为主，分别占总色素的23.8%、26.1%、14.7%和15.7%。16S rRNA基因和nifH基因的系统发育分析证实了菌株EIO409与蓝藻的亲缘关系较近。
One strain of unicellular greenish algae embedded by mucilage was successfully isolated from equatorial area in the Indian Ocean. Microscopic observation, ultrastructure features and genetic identification confirmed that the strain was closely related toCyanothecesp., which was a cyanobacteria species with great ecological significance. Cells were solitary with oval or bacilliform shapes. Diameters of this strain were relatively small, ranging from 2.5 to 6.5 μm on average. Ultrastructure of cells was simple. Thylakoids were arranged parietal and keritomized content were observed in the thylakoid region. Various electron-transparent granules with low electron-dense region as well as cyanophycin or glycogen granules-like organelle and carbonxysomes were also observed. For pigment composition, the dominant pigments were chlorophylla, β-Carotene, Zeaxanthin and an unknown pigment, contributing 23.8%, 26.1%, 14.7% and 15.7% to total pigments respectively. The phylogenetic analysis of 16S rRNA gene andnifH gene confirmed that Strain EIO409 was closely related withCyanothecesp..