OLIGONUCLEOTIDE DUPLEXES CONTAINING 2'-AMINO-2'-DEOXYCYTIDINES - THERMAL-STABILITY AND CHEMICAL-REACTIVITY
OLIGONUCLEOTIDE DUPLEXES CONTAINING 2'-AMINO-2'-DEOXYCYTIDINES - THERMAL-STABILITY AND CHEMICAL-REACTIVITY
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DOI：
10.1093/nar/22.1.20
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发表时间：
1994-01-11
影响因子：
14.9
通讯作者：
ECKSTEIN, F
中科院分区：
文献类型：
--
作者：
AURUP, H;TUSCHL, T;ECKSTEIN, F
测定了含有2 '-氨基-2'-脱氧胞苷的寡核苷酸的热稳定性，并与未修饰的寡核苷酸进行了比较。在pH 7和pH 5下，2 '-氨基糖苷的存在使RNA以及DNA环境中的双链体不稳定。通过C-13-NMR光谱测定2 ′-氨基的pK（a）为6.2。利用含有2 '-氨基糖苷的寡核苷酸的反应性，通过其异硫氰酸酯衍生物掺入罗丹明。
Thermal stabilities of oligonucleotides containing 2'-amino-2'-deoxycytidines were determined and compared to those of the unmodified oligonucleotides. The presence of the 2'-aminonucleoside destabilised duplexes in a RNA as well as a DNA context at pH 7 as well as at pH 5. The pK(a) of the 2'-amino group was determined by C-13-NMR spectroscopy to be 6.2. The reactivity of an oligonucleotide containing a 2'-aminonucleoside was exploited for the incorporation of rhodamine by its isothiocyanate derivative.