A metallic multisite recording system designed for continuous long-term monitoring of electrophysiological activity in slice cultures
A metallic multisite recording system designed for continuous long-term monitoring of electrophysiological activity in slice cultures
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DOI：
10.1016/s0956-5663(99)00015-9
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发表时间：
1999-04-30
影响因子：
12.6
通讯作者：
Corrèges, P
中科院分区：
文献类型：
--
作者：
Duport, S;Millerin, C;Corrèges, P
本文介绍了一种灵活的，金属多电极阵列，在卡普顿适合在记录室接口型器官型文化。该多位点记录系统设计用于连续多位点监测大鼠脑器官型骰子培养物的电生理活动。该系统由一个信号调节装置和一个包含微电极阵列的卡组成，该信号调节装置还掌握电刺激范例。该卡包括由刚性和可渗透膜封闭的灌注室，在该灌注室上放置支持切片培养物的穿孔微电极阵列。一旦用气体室封闭，卡的内部保持无菌且无污染，并且可以保持在培养箱内部或外部用于电生理分析。每个28镀金微电极记录位点的尺寸为50 μ m × 100 μ m。腔室和卡的设计使得可以在无菌条件下改变灌注介质和气体气氛，从而允许分析药理学化合物的长期作用。使用该阵列，可以执行切片的电活动的刺激和记录。用这种可重复使用的系统获得的信号表现出良好的信噪比。该装置进行了测试，以遵循在几天内相同的神经元组的诱发和/或自发电活动的演变和修改。(C)1999年Elsevier Science S.A. All rights reserved.
This paper describes a flexible, metallic multielectrode array, made on kapton to fit in a recording chamber for interface-type organotypic cultures. This multisite recording system is designed for continuous multisite monitoring of electrophysiological activity in rat brain organotypic dice cultures. The system is composed of a signal conditioning set-up, which also masters electrical stimulation paradigms and a card containing the microelectrode array. The card comprises a perfusion chamber closed by a rigid and permeable membrane on which the pierced microelectrode array supporting the slice culture is placed. Once closed with a gaseous chamber, the inside of the card remained sterile and free of contamination and could be maintained inside or outside the incubator for electrophysiological analyses. Dimensions of each 28-plated gold microelectrode recording site are 50 mu m x 100 mu m. The design of the chambers and the card makes it possible to modify both the perfusion medium and the gaseous atmosphere in sterile conditions, allowing thus analyses of long-term effects of pharmacological compounds. Using this array one can perform stimulation and recordings of the electrical activity of the slice. Signals obtained with this reusable system exhibit a good signal-to-noise ratio. This device was tested to follow the evolution and modifications of the evoked and/or spontaneous electrical activity of the same groups of neurones during several days. (C) 1999 Elsevier Science S.A. All rights reserved.