The antigen-specific, major histocompatibility complex-restricted receptor on T cells. VI. An antibody to a receptor allotype.
The antigen-specific, major histocompatibility complex-restricted receptor on T cells. VI. An antibody to a receptor allotype.
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DOI：
10.1084/jem.160.2.452
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发表时间：
1984-08-01
期刊：
影响因子：
--
通讯作者：
Marrack P
中科院分区：
文献类型：
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作者：
Haskins K;Hannum C;White J;Roehm N;Kubo R;Kappler J;Marrack P
我们用纯化的抗原受体加BALB/c T细胞杂交瘤DO-11.10的I-A免疫大鼠细胞，制备了单克隆抗体KJ 16 -133。与之前描述的大多数其他单克隆抗受体抗体不同，KJ 16 -133不是克隆特异性的。它与正常BALB/c小鼠T细胞上约20%的受体反应。它还与大约相同百分比的抗原特异性、主要组织相容性复合体（MHC）限制性或同种异体I区特异性T细胞杂交瘤反应。KJ 16 -133与给定的T细胞杂交瘤的反应似乎不依赖于所讨论的T细胞的抗原特异性或MHC限制元件。KJ 16 -133识别的行列式具有一些意想不到的性质。它在包括SJL/J和SJA/20在内的几种小鼠品系中不存在，但存在于大多数其他常用品系的T细胞上。因此，识别的决定子不映射到Igh。我们的实验表明，克隆特异性“抗独特型”抗体和KJ 16 -133识别受体不同部位的决定簇。例如，克隆特异性抗体与靶T细胞的结合对温度相对不敏感，而KJ 16 -133在37 ℃下与细胞结合良好，但在4 ℃下与细胞结合较差。由克隆特异性抗体识别的决定簇对受体的还原和烷基化敏感，而KJ 16 -133反应性不敏感。最后，饱和浓度的KJ 16 -133与靶T细胞的结合不阻断克隆特异性抗体的结合。类似地，克隆特异性抗体的结合仅略微抑制KJ 16 -133的结合。总而言之，这些结果表明KJ 16 -133针对的是T细胞上的等位基因决定簇，该决定簇可能靠近细胞膜，而不是抗原加MHC的受体结合位点。抗体可以识别恒定区同种型的等位基因或J区的等位基因。
We have prepared a monoclonal antibody, KJ16-133, from the cells of a rat immunized with the purified receptor for antigen plus I-A of a BALB/c T cell hybridoma, DO-11.10. Unlike most other monoclonal anti- receptor antibodies that have been described before, KJ16-133 is not clone specific. It reacts with approximately 20% of the receptors on T cells of normal BALB/c mice. It also reacts with about the same percentage of antigen-specific, major histocompatibility complex (MHC)- restricted or allogeneic I-region specific T cell hybridomas. Reaction of KJ16-133 with a given T cell hybridoma does not seem to depend on the antigen specificity or MHC-restricting element of the T cell in question. The determinant recognized by KJ16-133 has some unexpected properties. It is absent in several strains of mice including SJL/J and SJA/20, but present on the T cells of most other commonly used strains. The determinant recognized therefore does not map to Igh. Our experiments suggest that a clone-specific "antiidiotypic" antibody and KJ16-133 recognize determinants on different parts of the receptor. For example, the binding of a clone-specific antibody to target T cells is relatively temperature insensitive, whereas KJ16-133 binds well to cells at 37 degrees C but poorly to cells at 4 degrees C. The determinant recognized by a clone-specific antibody is sensitive to reduction and alkylation of the receptor, whereas KJ16-133 reactivity is not. Finally, binding of KJ16-133 at saturating concentrations to target T cells does not block the binding of a clone-specific antibody. Similarly, binding of a clone-specific antibody only marginally inhibits binding of KJ16-133. Taken together, these results suggest that KJ16-133 is directed against an allelic determinant on T cells that may be close to the membrane, and not in the receptor binding site for antigen plus MHC. The antibody may recognize an allele of a constant region isotype, or an allele of a J region.