Frequencies of memory T cells specific for varicella-zoster virus, herpes simplex virus, and cytomegalovirus by intracellular detection of cytokine expression
Frequencies of memory T cells specific for varicella-zoster virus, herpes simplex virus, and cytomegalovirus by intracellular detection of cytokine expression
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DOI：
10.1086/315347
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发表时间：
2000-03-01
影响因子：
6.4
通讯作者：
Arvin, AM
中科院分区：
文献类型：
--
作者：
Asanuma, H;Sharp, M;Arvin, AM
通过细胞内细胞因子（ICC）检测比较免疫成人中水痘带状疱疹病毒（VZV）、单纯疱疹病毒（HSV）和人巨细胞病毒（HCMV）特异性记忆T细胞。通过产生干扰素（IFN）-γ，VZV和HSV的CD 4（+）T细胞平均百分比分别为0.11%和0.22%; HCMV的频率显著更高，为1.21%，使用肿瘤坏死因子（TNF）-α，VZV-、HSV-和HCMV特异性CD 4（+）T细胞的计数相似。HCMV刺激的CD 8（+）T细胞产生IFN-γ（1.11%）和TNF-α（1.71%）;未检测到VZV和HSV特异性CD 8（+）T细胞。VZV CD 4（+）T细胞数量在具有自然或疫苗诱导免疫的年轻成人中相似。VZV CD 4（+）T细胞在老年人中的频率明显较低。通过ICC检测，二次水痘免疫并没有增加VZV特异性CD 4（+）T细胞的频率。疱疹病毒特异性记忆T细胞的数量可能随病毒潜伏期和年龄而变化。
Memory T cells specific for varicella-zoster virus (VZV), herpes simplex virus (HSV), and human cytomegalovirus (HCMV) were compared in immune adults by intracellular cytokine (ICC) detection. The mean percentages of CD4(+) T cells were 0.11% for VZV and 0.22% for HSV by interferon (IFN)-gamma production; the frequency for HCMV was significantly higher at 1.21%, Percentages of VZV-, HSV-, and HCMV-specific CD4(+) T cells were similar by use of tumor necrosis factor (TNF)-alpha. HCMV-stimulated CD8(+) T cells produced IFN-gamma (1.11%) and TNF-alpha (1.71%); VZV- and HSV-specific CD8(+) T cells were not detectable. VZV CD4(+) T cell numbers were similar in young adults with natural or vaccine-induced immunity. VZV CD4(+) T cells were significantly less frequent in older adults. Secondary varicella immunization did not increase VZV-specific CD4(+) T cell frequencies by ICC assay, Numbers of memory T cells specific for herpesviruses may vary with sites of viral latency and with hose age.