Effect of Mammalian Tissue Source on the Molecular and Macroscopic Characteristics of UV-Cured Type I Collagen Hydrogel Networks
Effect of Mammalian Tissue Source on the Molecular and Macroscopic Characteristics of UV-Cured Type I Collagen Hydrogel Networks
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DOI：
10.3390/prosthesis4010001
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发表时间：
2022-03-01
期刊：
影响因子：
3.4
通讯作者：
Tronci, Giuseppe
中科院分区：
文献类型：
--
作者：
Brooker, Charles;Tronci, Giuseppe
I型胶原蛋白的组织来源对于确保新医疗器械原型的可扩展性和监管友好型临床转化至关重要。然而，选择满足上述要求并符合新制造路线的胶原蛋白商业来源是具有挑战性的。本研究探讨了从三种不同的哺乳动物组织中提取的I型胶原蛋白对一种新的UV固化胶原蛋白水凝胶的分子和宏观特征的影响。选择胃蛋白酶溶解的牛去端肽胶原（BA）和胃蛋白酶溶解的猪去端肽胶原（PA）作为与不同安全性风险相关的市售原材料，并与来自大鼠尾部的内部酸提取I型胶原（CRT）进行比较。所有原料显示典型的二色性和电泳特征的I型胶原蛋白，而显着降低赖氨酸含量的PA样品上测量。在用4-乙烯基苄基氯（4VBC）共价官能化之后，BA和CRT产物产生具有显著增加的平均凝胶含量（G >= 97重量%）的相当的UV固化水凝胶，而猪变体显示出最高的溶胀比（SR = 2224 +/-242wt.%）以及压缩模量降低一个数量级（E-c = 6 +/-2kPa）。总的来说，这些结果支持使用牛组织作为化学上可行的I型胶原来源，用于实现具有竞争性机械性能和共价网络结构的UV固化水凝胶。
The tissue source of type I collagen is critical to ensure scalability and regulation-friendly clinical translation of new medical device prototypes. However, the selection of a commercial source of collagen that fulfils both aforementioned requirements and is compliant with new manufacturing routes is challenging. This study investigates the effect that type I collagen extracted from three different mammalian tissues has on the molecular and macroscopic characteristics of a new UV-cured collagen hydrogel. Pepsin-solubilised bovine atelocollagen (BA) and pepsin-solubilised porcine atelocollagen (PA) were selected as commercially available raw materials associated with varying safety risks and compared with in-house acid-extracted type I collagen from rat tails (CRT). All raw materials displayed the typical dichroic and electrophoretic characteristics of type I collagen, while significantly decreased lysine content was measured on samples of PA. Following covalent functionalisation with 4-vinylbenzyl chloride (4VBC), BA and CRT products generated comparable UV-cured hydrogels with significantly increased averaged gel content (G >= 97 wt.%), while the porcine variants revealed the highest swelling ratio (SR = 2224 +/- 242 wt.%) and an order of magnitude reduction in compression modulus (E-c = 6 +/- 2 kPa). Collectively, these results support the use of bovine tissues as a chemically viable source of type I collagen for the realisation of UV-cured hydrogels with competitive mechanical properties and covalent network architectures.