Novel evaluation method of dentin repair by direct pulp capping using high-resolution micro-computed tomography
Novel evaluation method of dentin repair by direct pulp capping using high-resolution micro-computed tomography
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DOI：
10.1007/s00784-018-2374-5
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发表时间：
2018-11-01
影响因子：
3.4
通讯作者：
Hayashi, Mikako
中科院分区：
文献类型：
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作者：
Okamoto, Motoki;Takahashi, Yusuke;Hayashi, Mikako
目的评价一种新的微型计算机断层扫描(Micro-CT)技术对牙本质修复的质量和数量的评估，该方法在标准化洞腔制备下直接盖髓后，组织学分析难以显示牙本质修复的质量和数量。材料与方法在Wistar大鼠上制备标准洞，并使用两种商用生物陶瓷ProRoot MTA和iRoot BP Plus进行直接盖髓。术后2周或4周，用高分辨率显微CT分析牙本质密度、牙本质矿物含量、第三牙本质致密性和完整性、牙本质有无空洞体积等指标，评价三级牙本质形成的质量和数量。结果iRoot BP Plus与ProRoot MTA的牙髓裸露面积大小相似。对两周后的样本进行的Micro-CT分析显示，iRoot BP Plus的第三代牙本质致密性显著高于ProRoot MTA(p<；0.05)。不含空隙的第三牙本质体积、牙本质矿物质含量和密度在两组间无显著差异。在4周的样本中，观察到iRoot BP Plus诱导的牙本质密度、致密性和牙本质体积显著增加(p<；0.05)。对三级牙本质完整性的显微CT分析显示，部分ProRoot MTA标本有较小的缺陷，缺乏连续性(6/512图像)。结论Micro-CT是一种准确、客观、全面的评价牙本质修复质量和数量的方法。
Objectives We evaluated a novel micro-computed tomography (micro-CT) assessment for quality and quantity of dentin repair, which is difficult to visualize by histological analysis, after direct pulp capping under standardized cavity preparation.Materials and methods Standardized cavities were prepared on Wistar rats and direct pulp capping was performed using two commercial bioceramics, ProRoot MTA, and iRoot BP Plus. After 2 or 4 weeks, quality and quantity of tertiary dentin formation were evaluated using high-resolution micro-CT analyses including dentin mineral density, dentin mineral contents, compactness and integrity of tertiary dentin, and dentin volume with/without void space. Reproducibility of micro-CTanalyses was confirmed by histological evaluation of the same specimen.Results The exposed pulp area sizes were similar between iRoot BP Plus and ProRoot MTA. Micro-CT analysis of 2-week samples showing compactness of tertiary dentin was significantly higher in iRoot BP Plus than ProRoot MTA (p < 0.05). Tertiary dentin volume without void space, dentin mineral contents, and density were not significantly different between the groups. In 4-week samples, a significant increase was observed in dentin mineral density, compactness, and dentin volume with/without void space induced by iRoot BP Plus (p < 0.05). Micro-CTanalysis of tertiary dentin integrity demonstrated that some ProRoot MTA specimens had small defects and lacked continuity (6/512 images). No defects were observed with iRoot BP Plus.Conclusions Micro-CT analysis was confirmed as an accurate, objective, and inclusive approach for evaluating quality and quantity of dentin repair.