The Cultivation of Arabidopsis for Experimental Research Using Commercially Available Peat-Based and Peat-Free Growing Media.
The Cultivation of Arabidopsis for Experimental Research Using Commercially Available Peat-Based and Peat-Free Growing Media.
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使用市售的基于泥炭和无泥炭的生长培养基来培养拟南芥进行实验研究。
DOI：
10.1371/journal.pone.0153625
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Dodd AN
中科院分区：
文献类型：
--
作者：
Drake T;Keating M;Summers R;Yochikawa A;Pitman T;Dodd AN
关于拟南芥的实验研究经常涉及到在堆肥或其他生长介质上栽培时对表型性状的量化。许多商业上可获得的生长基质都含有泥炭，但由于泥炭形成速度非常慢，提取泥炭是不可持续的。此外，泥炭的提取减少了泥炭地的生物多样性，并将储存的碳和甲烷释放到大气中。在这里，我们比较了拟南芥在基于泥炭的和几种商业上可获得的不含泥炭的生长介质(由椰子、堆肥树皮、木材纤维和家庭堆肥组成)上的实验性能，为在植物科学研究中减少泥炭的使用提供指导。在几种不含泥炭的栽培基质上栽培，会降低拟南芥的生物量积累和种子产量。拟南芥仅在Coir上的表现非常差，可能是因为这种介质完全不含硝酸盐。一些不含泥炭的栽培基质更容易受到真菌污染。我们发现，在我们的实验条件下，对照(以泥炭为基础的)生长基质的高压灭菌对我们检测的任何生理参数都没有影响。总体而言，我们得出结论，拟南芥在以泥炭为基础的生长基质上栽培时表现最好，因为当使用不含泥炭的基质时，种子产量几乎总是减少。这可能是因为拟南芥的标准实验室方案和生长条件针对泥炭基质进行了优化。然而，在营养生长阶段，一些表型性状在以泥炭为基础的和一些不含泥炭的基质上培养的植株之间是相似的，这表明在一定的条件下，无泥炭的基质可以适合于拟南芥的表型分析。
Experimental research involving Arabidopsis thaliana often involves the quantification of phenotypic traits during cultivation on compost or other growing media. Many commercially-available growing media contain peat, but peat extraction is not sustainable due to its very slow rate of formation. Moreover, peat extraction reduces peatland biodiversity and releases stored carbon and methane into the atmosphere. Here, we compared the experimental performance of Arabidopsis on peat-based and several types of commercially-available peat-free growing media (variously formed from coir, composted bark, wood-fibre, and domestic compost), to provide guidance for reducing peat use in plant sciences research with Arabidopsis. Arabidopsis biomass accumulation and seed yield were reduced by cultivation on several types of peat-free growing media. Arabidopsis performed extremely poorly on coir alone, presumably because this medium was completely nitrate-free. Some peat-free growing media were more susceptible to fungal contamination. We found that autoclaving of control (peat-based) growing media had no effect upon any physiological parameters that we examined, compared with non-autoclaved control growing media, under our experimental conditions. Overall, we conclude that Arabidopsis performs best when cultivated on peat-based growing media because seed yield was almost always reduced when peat-free media were used. This may be because standard laboratory protocols and growth conditions for Arabidopsis are optimized for peat-based media. However, during the vegetative growth phase several phenotypic traits were comparable between plants cultivated on peat-based and some peat-free media, suggesting that under certain circumstances peat-free media can be suitable for phenotypic analysis of Arabidopsis.