Parameterization of red blood cell elongation index - shear stress curves obtained by ektacytometry
Parameterization of red blood cell elongation index - shear stress curves obtained by ektacytometry
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DOI：
10.3109/00365510903266069
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发表时间：
2009-01-01
影响因子：
2.1
通讯作者：
Meiselman, Herbert J.
中科院分区：
文献类型：
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作者：
Baskurt, Oguz K.;Hardeman, Max R.;Meiselman, Herbert J.
通过旋转细胞测量法测量红细胞 (RBC) 变形能力，可得出一组在各种剪切应力 (SS) 下测量的伸长指数 (EI)，以 SS-EI 曲线或表格数据的形式呈现。这些对于详细分析很有用，但当需要对组之间的全局参数进行简单比较时可能不合适。根据SS-EI曲线的特征形状，提出了两种计算最大RBC伸长指数（EImax）和最大变形一半所需的剪切应力（SS1/2）的方法：（i）线性Lineweaver-Burke（LB）模型； (ii) Streekstra-Bronkhorst (SB) 模型。两种方法都有特定的假设，因此可能受到测量条件的影响。使用用不同浓度的戊二醛 (GA) 处理的红细胞和通过细胞计数法获得的数据，对 0.6-75 Pa 之间九个不同的 SS 范围比较了这两种方法。我们的结果表明：(i) 两种模型的灵敏度都可能受到 SS 范围和所采用的限制的影响； (ii) 在整个 SS 数据范围内，LB 模型的非线性曲线拟合方法比线性方法给出了更一致的结果； (iii) LB 方法更适合检测用 0.001-0.005% 戊二醛 (GA) 处理的红细胞和 40% 刚性细胞混合物之间的 SS1/2 差异，但对于 10% 刚性细胞的 SB 也同样敏感； (iv) EImax 的 LB 和 SB 方法对于 0.001% 和 0.003% GA 和 40% 刚性是等效的，其中 SB 对于 0.005% GA 更好，LB 对于 10% 刚性更好。
Measurement of red blood cell (RBC) deformability by ektacytometry yields a set of elongation indexes (EI) measured at various shear stresses (SS) presented as SS-EI curves, or tabulated data. These are useful for detailed analysis, but may not be appropriate when a simple comparison of a global parameter between groups is required. Based on the characteristic shape of SS-EI curves, two approaches have been proposed to calculate the maximal RBC elongation index (EImax) and the shear stress required for one-half of this maximal deformation (SS1/2): (i) linear Lineweaver-Burke (LB) model; (ii) Streekstra-Bronkhorst (SB) model. Both approaches have specific assumptions and thus may be subject to the measurement conditions. Using RBC treated with various concentrations of glutaraldehyde (GA) and data obtained by ektacytometry, the two approaches have been compared for nine different ranges of SS between 0.6-75 Pa. Our results indicate that: (i) the sensitivity of both models can be affected by the SS range and limits employed; (ii) over the entire range of SS-data, a non-linear curve fitting approach to the LB model gave more consistent results than a linear approach; (iii) the LB method is better for detecting SS1/2 differences between RBC treated with 0.001-0.005% glutaraldehyde (GA) and for a 40% mixture of rigid cells but is equally sensitive to SB for 10% rigid cells; and (iv) the LB and SB methods for EImax are equivalent for 0.001% and 0.003% GA and 40% rigid, with the SB better for 0.005% GA and the LB better for 10% rigid.