Interleukin-1β Stimulates Stromal-Derived Factor-1α Expression in Human Subacromial Bursa
Interleukin-1β Stimulates Stromal-Derived Factor-1α Expression in Human Subacromial Bursa
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DOI：
10.1002/jor.21416
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发表时间：
2011-11-01
影响因子：
2.8
通讯作者：
Bigliani, Louis U.
中科院分区：
文献类型：
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作者：
Blaine, Theodore A.;Cote, Mindy A.;Bigliani, Louis U.
肩峰下滑囊细胞产生的趋化因子在肩峰下滑囊炎和肩袖疾病中上调。我们假设法氏囊滑膜细胞中SDF-1 α的产生可能是由局部细胞因子如白细胞介素IL-1 β和IL-6诱导的。肩峰下囊标本取自接受肩部手术的患者。法氏囊标本通过免疫组织化学染色，用抗人IL-1、IL-6和SDF-1 α抗体染色，并与正常和类风湿对照进行比较。法氏囊细胞也从标本中分离并培养。然后用细胞因子（IL-1 β和IL-6）处理早期传代细胞，用ELISA和RT-PCR检测SDF-1 α的表达。与正常对照相比，人肩峰下滑囊炎标本中SDF-1 α、IL-1 β和IL-6的表达水平较高。在培养的法氏囊滑膜细胞中，IL-1 β处理的细胞上清液中SDF-1 α的产生呈剂量依赖性增加。在IL-1 β处理的法氏囊细胞中，SDF-1 α mRNA的表达也增加。IL-6引起SDF-1 α表达的微小但无统计学意义的增加。SDF-1 α、IL-1 β和IL-6在肩峰下滑囊炎患者炎症的人肩峰下滑囊组织中表达。在培养的法氏囊滑膜细胞中，IL-1 β可刺激SDF-1 α基因表达和蛋白生成。人肩峰下法囊的滑膜细胞和炎症细胞产生的IL-1 β是发生在肩峰下法囊炎和肩袖疾病的炎症反应中的一个重要信号。(C) 2011骨科研究学会。Wiley期刊公司出版。[J]中华口腔医学杂志，2011
Chemokines produced by synoviocytes of the subacromial bursa are up-regulated in subacromial bursitis and rotator cuff disease. We hypothesized that SDF-1 alpha production in bursal synoviocytes may be induced by local cytokines such as interleukin IL-1 beta and IL-6. Subacromial bursa specimens were obtained from patients undergoing shoulder surgery. Bursal specimens were stained with anti-human antibodies to IL-1, IL-6, and SDF-1 alpha by immunohistochemistry and compared to normal and rheumatoid controls. Bursal cells were also isolated from specimens and cultured. Early passaged cells were then treated with cytokines (IL-1 beta and IL-6) and SDF-1 alpha expression was measured by ELISA and RT-PCR. SDF-1 alpha, IL-1 beta, and IL-6 were expressed at high levels in bursitis specimens from human subacromial bursa compared to normal controls. In cultured bursal synoviocytes, there was a dose-dependent increase in SDF-1 alpha production in the supernatants of cells treated with IL-1 beta. SDF-1 alpha mRNA expression was also increased in bursal cells treated with IL-1 beta. IL-6 caused a minimal but not statistically significant increase in SDF-1 alpha expression. SDF-1 alpha, IL-1 beta, and IL-6 are expressed in the inflamed human subacromial bursal tissues in patients with subacromial bursitis. In cultured bursal synoviocytes, SDF-1 alpha gene expression and protein production are stimulated by IL-1 beta. IL-1 beta produced by bursal syvoviocytes and inflammatory cells in the human subacromial bursa is an important signal in the inflammatory response that occurs in subacromial bursitis and rotator cuff disease. (C) 2011 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29:1695-1699, 2011