cDNA microarray analysis of gene expression during Fe-deficiency stress in barley suggests that polar transport of vesicles is implicated in phytosiderophore secretion in Fe-deficient barley roots
cDNA microarray analysis of gene expression during Fe-deficiency stress in barley suggests that polar transport of vesicles is implicated in phytosiderophore secretion in Fe-deficient barley roots
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DOI：
10.1046/j.1365-313x.2002.01270.x
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发表时间：
2002-04-01
期刊：
影响因子：
7.2
通讯作者：
Nishizawa, NK
中科院分区：
文献类型：
--
作者：
Negishi, T;Nakanishi, H;Nishizawa, NK
为了从土壤中获取铁，禾本科植物从其根部分泌麦黄酸家族植物铁载体（MA）。铁缺乏时，MAs的分泌增加，并表现出明显的昼夜节律。我们使用了一个包含8987个水稻EST克隆cDNA的微阵列来研究缺铁胁迫下大麦根中的基因表达谱。大约200个克隆被鉴定为铁缺乏诱导基因，其中7个以前已经确定。为了满足生产MA对蛋氨酸的需求增加，铁缺乏增强了参与蛋氨酸合成的基因的表达，以及通过阳循环回收蛋氨酸。在这200个基因中，约50个在缺铁根中中午和晚上表现出不同的转录水平。时间过程实验的北方印迹分析证实，这些基因中的五个表现出其表达水平的昼夜变化。这些基因表达的昼夜变化表明，极性囊泡运输参与了MAs的昼夜分泌。
To acquire Fe from soil, graminaceous plants secrete mugineic acid family phytosiderophores (MAs) from their roots. The secretion of MAs increases in response to Fe deficiency, and shows a distinct diurnal rhythm. We used a microarray that included 8987 cDNAs of rice EST clones to examine gene expression profiles in barley roots during Fe-deficiency stress. Approximately 200 clones were identified as Fe-deficiency-inducible genes, of which seven had been identified previously. In order to meet the increased demand for methionine to produce MAs, Fe-deficiency enhances the expression of genes that participate in methionine synthesis, as well as recycling methionine through the Yang cycle. Of these 200 genes, approximately 50 exhibited different transcription levels in Fe-deficient roots at noon and at night. Northern blot analysis of time course experiments confirmed that five of these genes exhibited a diurnal change in their level of expression. The diurnal changes in the expression of these genes suggest that polar vesicle transport is involved in the diurnal secretion of MAs.