Waltonitone induces human hepatocellular carcinoma cells apoptosis in vitro and in vivo.
Waltonitone induces human hepatocellular carcinoma cells apoptosis in vitro and in vivo.
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DOI：
10.1016/j.canlet.2009.05.023
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发表时间：
2009-12
期刊：
影响因子：
9.7
通讯作者：
Zhang Zhang-Zhang;Shuo Wang;Hong-Hong Qiu-Hong;C. Duan;K. Ding;Zhengtao Wang
中科院分区：
文献类型：
--
作者：
Zhang Zhang-Zhang;Shuo Wang;Hong-Hong Qiu-Hong;C. Duan;K. Ding;Zhengtao Wang
华托尼酮（Waltonitone）是一种新的熊烷型五环三萜化合物，它能显著抑制人肝癌BEL-7402细胞的生长。AO/EB染色和流式细胞术检测细胞凋亡。细胞凋亡基因芯片检测结果显示BCL-2家族基因可能在细胞凋亡中起重要作用。RT-PCR和Western blotting分析表明，Waltonitone可通过死亡受体途径和线粒体途径诱导肿瘤细胞凋亡。同时，采用BEL-7402肿瘤细胞裸鼠移植瘤模型，观察了华托尼酮的体内抑制作用。综上所述，这些研究表明，在体外和体内，Waltonitone可以抑制肝癌细胞的生长和诱导凋亡。
Waltonitone, a new ursane-type pentacyclic triterpene isolated from Gentian waltonii Burkill significantly inhibited human hepatocellular carcinoma BEL-7402 cells growth. Apoptosis induced by waltonitone was characterized by AO/EB staining and flow cytometric analysis. Apoptosis microarray assay results showed BCL-2 family genes might especially play an important role in waltonitone-induced apoptosis. RT-PCR and Western blotting analysis showed that waltonitone could induce tumor cell apoptosis via both death receptor and mitochondria pathways. Meanwhile, the inhibitory effect of waltonitone was examined in vivo using BEL-7402 tumor cells xenografted into athymic mice model. In summary, these studies demonstrated that waltonitone might inhibit hepatocellular carcinoma cells growth and induce apoptosis in vitro and in vivo.