Non-stationary fluctuation analysis of macroscopic gap junction channel records.
Non-stationary fluctuation analysis of macroscopic gap junction channel records.
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宏观间隙连接通道记录的非平稳波动分析。
DOI：
10.1007/s00232-005-0765-4
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Brink,PR
中科院分区：
文献类型：
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作者：
Ramanan,SV;Valiunas,V;Brink,PR
非平稳波动分析应用于宏观记录的连接电流所产生的同型Cx37和Cx43间隙连接通道在RIN细胞中表达。现有的分析方法，考虑到地方病解偶联的修改，数据进行了分析。结果是一致的，两个通道具有开放的概率范围从0.7到接近统一的低跨接电压。该分析还产生了两种通道类型的单通道电导的估计值，类似于单通道记录中所见。这里提出的结果表明，波动分析可以用来提取宏观双全细胞记录的单通道间隙连接电导。这些结果也构成了经验确定的估计开放的概率，不依赖于模型。
Non-stationary fluctuation analysis was applied to macroscopic records of junctional currents arising from homotypic Cx37 and Cx43 gap junction channels expressed in RIN cells. The data were analyzed by a modification of existing analytical methods that takes endemic uncoupling into account. The results are consistent with both channels having open probabilities ranging from 0.7 to near unity for low transjunctional voltages. The analysis also yielded estimates of single-channel conductances for the two channel types similar to those seen in single-channel recordings. The results presented here show that fluctuation analysis can be used to extract single-channel gap junctional conductances from macroscopic double whole-cell recordings. These results also constitute empirically determined estimates of the open probability that are not model-dependent.