hASH1 expression is closely correlated with endocrine phenotype and differentiation extent in pulmonary neuroendocrine tumors
hASH1 expression is closely correlated with endocrine phenotype and differentiation extent in pulmonary neuroendocrine tumors
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DOI：
10.1038/modpathol.3800038
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发表时间：
2004-02-01
期刊：
影响因子：
7.5
通讯作者：
Okayasu, I
中科院分区：
文献类型：
--
作者：
Jiang, SX;Kameya, T;Okayasu, I
果蝇神经源性无毛鳞片基因hASH 1的人类同源物1在胎儿肺神经内分泌细胞和一些神经内分泌肿瘤细胞系中特异性表达。然而，尚未收集到关于其在肿瘤中的体内表达的数据。应用原位杂交技术检测238例手术切除肺癌组织中hASH 1 mRNA的表达，并分析hASH 1表达状态与神经内分泌标志物嗜铬粒蛋白A、神经细胞粘附分子、胃泌素释放肽和降钙素免疫组化结果及临床预后的相关性。hASH 1在2/20（10%）腺癌、4/30（10%）腺癌和4/30（10%）腺癌中表达。（13.3%）典型类癌，11/13不典型类癌38例（84.6%）大细胞神经内分泌癌56.7%，（71.8%）的小细胞癌，但没有在任何鳞状细胞癌（0/21）或大细胞癌（0/9）。2例hASH 1（+）腺癌也表达多种神经内分泌标志物。因此，hASH 1表达仅限于具有神经内分泌表型的肺癌。然而，并非所有的神经内分泌肿瘤表达hASH 1。在大细胞神经内分泌癌和小细胞癌中，hASH 1表达与嗜铬粒蛋白A、胃泌素释放肽和降钙素表达密切相关（P
The human homolog 1 of the Drosophila neurogenic achaete-scute genes, hASH1, is specifically expressed in fetal pulmonary neuroendocrine cells and in some neuroendocrine tumor cell lines. However, no data have been gathered regarding its in vivo expression in tumors. hASH1 mRNA expression was investigated by in situ hybridization in 238 surgically resected lung carcinomas, and the correlations between hASH1 expression status and immunostaining results of neuroendocrine markers chromogranin A, neural cell adhesion molecule, gastrin-releasing peptide and calcitonin, and clinical outcome were analyzed. hASH1 expression was detected in 2/20 (10%) adenocarcinomas, 4/30 (13.3%) typical carcinoids, 11/13 (84.6%) atypical carcinoids, 38/67 (56.7%) large-cell neuroendocrine carcinomas and 56/78 (71.8%) small-cell carcinomas, respectively, but not in any squamous cell carcinoma (0/21) or large-cell carcinoma (0/9). The 2 hASH1(+) adenocarcinomas also expressed multiple neuroendocrine markers. Thus, hASH1 expression was restricted to lung cancers with neuroendocrine phenotypes. However, not all neuroendocrine tumors expressed hASH1. Within the entities of large-cell neuroendocrine carcinoma and small-cell carcinoma, hASH1 expression correlated very closely with chromogranin A, gastrin-releasing peptide and calcitonin expression (P