SIRT1 Protects Against Apoptosis by Promoting Autophagy in the Oxygen Glucose Deprivation/Reperfusion-Induced Injury
SIRT1 Protects Against Apoptosis by Promoting Autophagy in the Oxygen Glucose Deprivation/Reperfusion-Induced Injury
复制标题
SIRT1 通过促进氧葡萄糖剥夺/再灌注引起的损伤中的自噬来防止细胞凋亡
DOI：
10.3389/fneur.2019.01289
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发表时间：
2019-12-05
影响因子：
3.4
通讯作者：
Fang, Marong
中科院分区：
文献类型：
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作者：
Ren, Qiannan;Hu, Zhiying;Fang, Marong
沉默信息调节因子1（SIRT 1）有助于细胞调节。已有研究报道SIRT 1在大鼠脑缺血再灌注损伤模型缺血半暗带区异常表达。我们研究了SIRT 1对氧和葡萄糖剥夺/再灌注（OGD/R）细胞损伤的影响。将过表达或沉默的SIRT 1嗜铬细胞瘤12（PC 12）细胞暴露于体外OGD/R损伤。Western blot、TUNEL染色和免疫荧光分析检测细胞凋亡和自噬。我们发现，自噬和凋亡被上调和下调，分别在OGD/R诱导的PC 12细胞中SIRT 1的过表达。我们还发现SIRT 1的沉默分别导致自噬和凋亡的下调和上调。基于以上结果，我们推测SIRT 1在体外能够促进自噬和抑制凋亡，从而对OGD/R诱导的损伤具有潜在的神经保护作用。这可能有助于脑I/R损伤治疗方法的发展。
Silent information regulator 1 (SIRT1) contributes to cellular regulation. Previous studies have reported SIRT1 to be abnormally expressed in the ischemic penumbra of cerebral ischemia/reperfusion (I/R) injury rat model. We investigated the effect of SIRT1 on oxygen and glucose deprivation/reperfusion (OGD/R) cell injury. Over-expressed or silenced SIRT1 pheochromocytoma 12 (PC12) cells were exposed to an in-vitro OGD/R injury. Western blot, TUNEL staining and immunofluorescence analyses were performed to assess apoptosis and autophagy. We found autophagy and apoptosis to be up-regulated and down-regulated, respectively, following the over-expression of SIRT1 in the OGD/R-induced PC12 cells. We also found the silencing of SIRT1 to culminate in the down-regulation and up-regulation of autophagy and apoptosis, respectively. On the basis of our results, we surmise that SIRT1 can promote autophagy and inhibit apoptosis in-vitro, and thus exhibit potential neuroprotection against OGD/R-induced injury. This could facilitate in the development of therapeutic approaches for cerebral I/R injury.