GLUTAMATE IONTOPHORESIS INDUCES LONG-TERM POTENTIATION IN THE ABSENCE OF EVOKED PRESYNAPTIC ACTIVITY
GLUTAMATE IONTOPHORESIS INDUCES LONG-TERM POTENTIATION IN THE ABSENCE OF EVOKED PRESYNAPTIC ACTIVITY
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DOI：
10.1016/0896-6273(93)90206-7
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发表时间：
1993-05-01
期刊：
影响因子：
16.2
通讯作者：
KELLY, PT
中科院分区：
文献类型：
--
作者：
CORMIER, RJ;MAUK, MD;KELLY, PT
Protocols that induce long-term potentiation (LTP) typically involve afferent stimulation. We tested the hypothesis that LTP induction does not require presynaptic activity. The significance of this hypothesis is underscored by results suggesting that LTP expression may involve activity-dependent presynaptic changes. An induction protocol using glutamate iontophoresis was developed that reliably induced LTP in hippocampal slices without afferent stimulation. Iontophoresis LTP was Ca2+ dependent, was blocked by MK-801, and occluded tetanus-induced LTP. Iontophoresis LTP was induced when excitatory postsynaptic potentials were completely blocked by adenosine plus tetrodotoxin. Our results suggest constraints on the involvement of presynaptic mechanisms and putative retrograde messengers in LTP induction and expression; namely, these processes must function without many forms of activity-dependent presynaptic processes.
