Pre-exercise branched chain amino acid administration increases endurance performance in rats
Pre-exercise branched chain amino acid administration increases endurance performance in rats
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DOI：
10.1097/00005768-199709000-00010
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发表时间：
1997-09-01
期刊：
影响因子：
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通讯作者：
Lacroix, EM
中科院分区：
文献类型：
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作者：
Calders, P;Pannier, JL;Lacroix, EM
本研究探讨了运动前给予支链氨基酸（BCAA）对大鼠跑台运动时血氨水平和力竭时间的影响。成年雌性Wistar大鼠在电动跑步机上训练。禁食24小时后，将大鼠腹腔内（i. p.）在进行30分钟次极量运动（N = 18）或跑步至力竭（N = 12）前5分钟，给予1 mL安慰剂或BCAA（30 mg）。在这两种情况下，大鼠在运动后立即处死，并收集血液用于测量葡萄糖、非酯化脂肪酸（NEFA）、乳酸、支链氨基酸、氨和游离色氨酸（游离TRP）水平。从接受相同处理和程序的久坐大鼠（N = 30）中获得对照值。血浆BCAA水平增加三倍后5分钟内BCAA管理。与安慰剂组（76 ± 4分钟）相比，服用BCAA组（99 ± 9分钟）的平均跑步至力竭时间显著延长（P < 0.01）。在运动过程中，血氨水平明显高于BCAA组大鼠在30 min运动时与安慰剂组比较，差异有显著性（P < 0.01）（113 +/- 25 μ mol.L-1（BCAA）vs 89 +/- 16 μ mol.L-1）和运动至力竭后（186 +/- 44 μ mol.L-1（BCAA）vs 123 +/- 19 μ mol.L-1）。这些数据表明，大鼠服用BCAA可增强耐力表现，并增加运动期间的血氨。
This study investigated the effects of pre-exercise branched-chain amino acid (BCAA) administration on blood ammonia levels and on time to exhaustion during treadmill exercise in rats. Adult female Wistar rats were trained on a motor driven treadmill. After a 24-h fast, rats were injected intraperitoneally (i.p.) with 1 mL of placebo or BCAA (30 mg), 5 min before performing 30 min of submaximal exercise (N = 18) or running to exhaustion (N = 12). In both cases, rats were sacrificed immediately following exercise, and blood was collected for the measurement of glucose, nonesterified fatty acid (NEFA), lactic acid, BCAA, ammonia, and free-tryptophan (free-TRP) levels. Control values were obtained from sedentary rats that were subjected to identical treatments and procedures (N = 30). Plasma BCAA levels increased threefold within 5 min after BCAA administration. Mean run time to exhaustion was significantly longer (P < 0.01) after BCAA administration (99 +/- 9 min) compared with placebo (76 +/- 4 min). During exercise, blood ammonia levels were significantly higher (P < 0.01) in the BCAA treated compared with those in the placebo treated rats both in the 30-min exercise bout (113 +/- 25 mu mol.L-1 (BCAA) vs 89 +/- 16 mu mol.L-1) and following exercise to exhaustion (186 +/- 44 mu mol.L-1 (BCAA) vs 123 +/- 19 mu mol.L-1). These data demonstrate that BCAA administration in rats results in enhanced endurance performance and an increase in blood ammonia during exercise.