Stimulation by human placental conditioned medium of hemopoietic colony formation by human marrow cells.
Stimulation by human placental conditioned medium of hemopoietic colony formation by human marrow cells.
复制标题
人胎盘条件培养基刺激人骨髓细胞形成造血集落。
作者：
A. Burgess;E. M. Wilson;D. Metcalf
在半固体琼脂培养中，人胎盘组织条件培养液能刺激人骨髓细胞形成粒细胞和单核细胞集落。普通条件培养液的集落刺激活性与标准外周血底层的活性相当。胎盘是活性物质的可靠来源，一个胎盘提供的材料足以刺激5,000-10,000个正常或白血病细胞培养。采用硫酸铵沉淀、磷酸钙凝胶吸附、DEAE-纤维素批吸附、Sephadex G-150凝胶过滤、聚丙氨酰胺凝胶电泳法，将人胎盘条件培养液(CSFHPCM)中的集落刺激因子浓缩纯化1800倍。该活性因子在凝胶过滤中表现为一种大分子，表观分子量为30,000道尔顿。胎盘条件培养液中的活性因子不依赖于具有内源性集落刺激活性的贴壁骨髓细胞的存在。
Medium conditioned by human placental tissue was found to stimulate granulocytic and monocytic colony formation by human marrow cells in semisolid agar cultures. The colony-stimulating activity of unfractionated conditioned medium was equivalent to the activity of standard peripheral blood underlayers. Placentas were a reliable source of active material, and one placenta provided enough material to stimulate 5,000-10,000 cultures of normal or leukemic cells. The colony-stimulating factor in human placental conditioned medium (CSFHPCM) was concentrated and purified 1800-fold using ammonium sulfate precipitation, calcium phosphate gel absorption, DEAE-cellulose batch absorption, gel filtration on Sephadex G-150, and polyarcylamide gel gel electrophoresis. The active factor behaved on gel filtration as a macromolecule with an apparent molecular weight of 30,000 daltons. The active factor in placental conditioned medium was not dependent on the presence of adherent marrow cells with endogenous colony-stimulating activity.