INFLUENCE OF ANATOMIC SITE AND AGE ON THE REPLICATION AND DIFFERENTIATION OF RAT ADIPOCYTE PRECURSORS IN CULTURE
INFLUENCE OF ANATOMIC SITE AND AGE ON THE REPLICATION AND DIFFERENTIATION OF RAT ADIPOCYTE PRECURSORS IN CULTURE
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DOI：
10.1172/jci111075
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发表时间：
1983-01-01
影响因子：
15.9
通讯作者：
HOLLENBERG, CH
中科院分区：
文献类型：
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作者：
DJIAN, P;RONCARI, DAK;HOLLENBERG, CH
使用70-400 g大鼠脂肪细胞前体的增殖细胞培养系统，我们探讨了脂肪组织特性的区域变化可能反映细胞固有的特定位点特征，而不是细胞外影响的可能性。最初，研究是由肾周脂肪组织间质的成纤维细胞样细胞的性质。使用集落形成技术，显示这些细胞是脂肪细胞前体;来自单个细胞的每个汇合集落显示分化的脂肪细胞。这种特性在所有年龄大鼠的细胞中都很明显，并在二次培养期间持续存在。在所有年龄的大鼠研究中，肾周细胞比附睾前体细胞复制得更快，对于179-g大鼠，群体倍增时间为19.3 +/- 0.7 vs. 25.5 +/- 1.2 h（平均值+/- SEM，P小于0.03）。随着年龄的增长，肾周细胞的复制率逐渐下降。在克隆条件下，肾周和附睾前体细胞的殖民地大小分布显示其复制能力的异质性，肾周细胞表现出更大的增殖。这些也分化更广泛的形态和酶的标准。年龄和地点的影响，持续通过许多细胞代;然而，高脂肪喂养没有永久的影响。与附睾前体相比，肾周的不同性质可能反映了基因表达调控的差异。这些数据也与肾周组织胚胎学生命的后期发展相一致。我们认为，脂肪细胞前体池的组成是一个重要的决定因素的脂肪组织的生长，发生在响应营养负荷。组成的区域间或个体间差异可以解释脂肪积累的区域和个体差异。图片
Using a propagating cell culture system of adipocyte precursors from 70-400-g rats, we explored the possibility that regional variations in properties of adipose tissue may reflect site-specific characteristics intrinsic to the cells, rather than extracellular influences. Initially, studies were made of the nature of the fibroblastlike cells from perirenal adipose tissue stroma. Using colony-forming techniques, it was shown that these cells were adipocyte precursors; each confluent colony that was derived from a single cell displayed differentiated adipocytes. This characteristic was evident in cells from rats of all ages and persisted during secondary culture. At all ages of rats studied, perirenal cells replicated more rapidly than epididymal precursors, e.g., for 179-g rats the population-doubling times were 19.3 +/- 0.7 vs. 25.5 +/- 1.2 h (means +/- SEM, P less than 0.03). With aging of the rats, the replication rate of their perirenal cells decreased progressively. Under clonal conditions, the colony size distribution of both perirenal and epididymal precursors revealed heterogeneity in their capacity for replication, perirenal cells showing greater proliferation. These also differentiated more extensively by morphologic and enzymatic criteria. Age and site had effects that persisted through many cell generations; however, high-fat feeding had no perpetuating influence. The dissimilar properties of perirenal as compared with epididymal precursors may reflect differences in regulation of gene expression. The data are also compatible with a later development in embryological life of perirenal tissue. We suggest that the composition of the adipocyte precursor pool is an important determinant of the growth of adipose tissue that occurs in response to a nutrient load. Interregional or interindividual variation in composition may explain regional and individual differences in fat accumulation.Images