Multi-Spectral Two-Photon Excited Fluorescence Microscopy Using Supercontinuum Light Source
Multi-Spectral Two-Photon Excited Fluorescence Microscopy Using Supercontinuum Light Source
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DOI：
10.1143/jjap.44.l167
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发表时间：
2005-01
影响因子：
1.5
通讯作者：
K. Isobe;W. Watanabe;S. Matsunaga;T. Higashi;K. Fukui;K. Itoh
中科院分区：
文献类型：
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作者：
K. Isobe;W. Watanabe;S. Matsunaga;T. Higashi;K. Fukui;K. Itoh
报道了一种利用宽带超连续谱光进行多光谱双光子激发荧光显微术的新技术。利用钛宝石飞秒振荡器在4.5mm长的光子晶体光纤中产生了近红外超连续谱。超连续谱作为双光子激发荧光显微镜的多光谱激发光源。我们证明，细胞中的细胞器的三色荧光图像可以同时获得。
We report on a novel technique of multi-spectral two-photon-excited fluorescence microscopy by use of broadband supercontinuum light. A supercontinuum in the near-infrared region is generated in a 4.5-mm-long photonic crystal fiber using a Ti:sapphire femtosecond oscillator. The supercontinuum is used as a multi-spectral excitation light source in a two-photon excited fluorescence microscope. We demonstrate that three-color fluorescence images of organelles in a cell can be simultaneously acquired.