Preparation of a Ruthenium-Complex-Functionalized Two-Photon-Excited Red Fluorescence Silicon Nanoparticle Composite for Targeted Fluorescence Imaging and Photodynamic Therapy in Vitro
Preparation of a Ruthenium-Complex-Functionalized Two-Photon-Excited Red Fluorescence Silicon Nanoparticle Composite for Targeted Fluorescence Imaging and Photodynamic Therapy in Vitro
复制标题
用于体外靶向荧光成像和光动力治疗的钌络合物功能化双光子激发红色荧光硅纳米粒子复合材料的制备
DOI：
10.1021/acsami.9b00288
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发表时间：
2019-04-17
影响因子：
9.5
通讯作者：
Zhang, Yu-Kui
中科院分区：
文献类型：
--
作者：
Dou, Ya-Kun;Shang, Yue;Zhang, Yu-Kui
Silicon nanoparticles (SiNPs), especially those emitting red fluorescence, have been widely applied in the field of bioimaging. However, harsh synthetic conditions and strong biological autofluorescence caused by short wavelength excitation restrict the further development of SiNPs in the field of biological applications. Here, we report a method for synthesizing a ruthenium-complex-functionalized two-photon excited red fluorescence silicon nanoparticle composite (SiNPs Ru) based on fluorescence resonance energy transfer under mild experimental conditions. In the prepared SiNPs Ru composite, silicon nanoparticles synthesized by atmospheric pressure microwave-assisted synthesis served as a fluorescence energy donor, which had two-photon fluorescence properties, and tris(4,4'-dicarboxylic acid-2,2-bipyridyl)ruthenium(II) dichloride (LRu) acted as a fluorescence energy acceptor, which could emit red fluorescence as well as had the ability to produce singlet-oxygen for photodynamic therapy. Therefore, the synthesized SiNPs Ru could emit red fluorescence by two-photon excitation based on fluorescence resonance energy transfer, which could effectively avoid the interference of biological autofluorescence. Fluorescence imaging tests in zebrafish and nude mice indicated that the as-prepared SiNPs Ru could act as a new kind of fluorescence probe for fluorescence imaging in vivo. By coupling folic acid (FA) to SiNPs Ru, the prepared composite (FA SiNPs Ru) could not only serve as a targeted two photon fluorescence imaging probe but also kill cancer cells via photodynamic therapy in vitro.
