Bcl2-negative follicular lymphomas frequently have Bcl6 translocation and/or Bcl6 or p53 expression
Bcl2-negative follicular lymphomas frequently have Bcl6 translocation and/or Bcl6 or p53 expression
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DOI：
10.1111/j.1440-1827.2006.02072.x
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发表时间：
2007-03-01
影响因子：
2.2
通讯作者：
Ohshima, Koichi
中科院分区：
文献类型：
--
作者：
Guo, Ying;Karube, Kennosuke;Ohshima, Koichi
Bcl 2是参与滤泡性淋巴瘤（FL）发病机制的重要蛋白质。然而，大约10%的FL病例不表达Bcl 2。本研究旨在比较Bcl-2阳性和阴性FL病例的基因畸变、促生存基因表达、细胞凋亡和增殖率。采用荧光原位杂交（FISH）技术检测并比较Bcl 2和Bcl 6易位。Bcl-2阴性FL较Bcl-2阳性FL易发生Bcl-6易位。免疫组化法检测Bcl-X、BAX、p53、Bcl 6的表达。Bcl-2家族蛋白Bcl-X和BAX在两种FL类型中表达相似。Bcl-2阴性的FL中，Bcl-6和p53蛋白均呈高表达，而Bcl-2阳性的FL中未见。Bcl 6与p53的表达呈负相关。这些结果提示Bcl 6易位在Bcl 2阴性FL中发生率更高，而且其他促生存蛋白如p53和Bcl 6可能在Bcl 2阴性FL的发病中起重要作用。
Bcl2 is an important protein involved in the pathogenesis of follicular lymphoma (FL). However, approximately 10% of FL cases do not express Bcl2. The present study was designed to compare gene aberrations, prosurvival gene expression, apoptosis and proliferation rates in Bcl2-positive and -negative FL cases. Bcl2 translocation and Bcl6 translocation were detected and compared using fluorescence in situ hybridization (FISH). A tendency for Bcl6 translocation to occur was found more frequently in Bcl2-negative FL than in the Bcl2-positive cases. The expression of Bcl-X, BAX, p53, Bcl6 was analyzed by immunohistochemistry. Bcl2 family proteins Bcl-X and BAX were expressed similarly in the two FL types. In some cases of Bcl2-negative FL there was high expression of Bcl6 or p53 but no such Bcl2-positive FL cases were detected. Furthermore, there was an inverse relationship between the expression of Bcl6 and p53. These results indicate that the Bcl6 translocation occurs more frequently in Bcl2-negative FL. Furthermore, other prosurvival proteins such as p53 and Bcl6 may play an important role in the pathogenesis of Bcl2-negative FL.