Rapid determination of methyl mercury in fish and shellfish: method development.
Rapid determination of methyl mercury in fish and shellfish: method development.
复制标题
鱼类和贝类中甲基汞的快速测定：方法开发。
DOI：
10.1093/jaoac/70.1.24
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
M. T. Corcoran
中科院分区：
文献类型：
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作者：
S. Hight;M. T. Corcoran
AOAC对鱼类和贝类中甲基汞的官方第一行动方法进行了修改，以提供更快速的测定。通过加入HCl并用甲苯萃取生成的氯化甲基汞，从均质化的丙酮洗涤组织中分离出甲基汞。在用氯化汞溶液处理的5% DEGS-PS上通过电子捕获气相色谱（GC）分析提取物。该方法的定量限为0.25微克Hg/g。采用AOAC官方第一行动法分析了箭鱼、鲨鱼、金枪鱼、虾、蛤、牡蛎和国家统计局研究材料-50（金枪鱼）中的甲基汞。所有产品还采用改良方法和AOAC官方方法分析了总汞。此外，选定的提取物与改进的方法进行了分析，通过气相色谱与汞选择性，微波诱导氦等离子体检测。各种方法的结果之间无显著差异。基本上所有存在的汞（以总汞计）都是有机形式。对于甲基汞含量为0.50-2.30微克Hg/g的鱼类和贝类，采用改良方法进行分析得出的变异系数为1%至7%。总平均回收率为100.5%。
The AOAC official first action method for methyl mercury in fish and shellfish was modified to provide more rapid determination. Methyl mercury is isolated from homogenized, acetone-washed tissue by addition of HCl and extraction by toluene of the methyl mercuric chloride produced. The extract is analyzed by electron capture gas chromatography (GC) on 5% DEGS-PS treated with mercuric chloride solution. The quantitation limit of the method is 0.25 micrograms Hg/g. Swordfish, shark, tuna, shrimp, clams, oysters, and NBS Research Material-50 (tuna) were analyzed for methyl mercury by the AOAC official first action method. All products also were analyzed by the modified method and the AOAC official method for total Hg. In addition, selected extracts obtained with the modified method were analyzed by GC with Hg-selective, microwave-induced helium plasma detection. There was no significant difference between the results for the various methods. Essentially all the Hg present (determined as total Hg) was in the organic form. Coefficients of variation from analyses by the modified method ranged from 1 to 7% for fish and shellfish containing methyl mercury at levels of 0.50-2.30 micrograms Hg/g. The overall average recovery was 100.5%.