Placental Growth Hormone and Lactogen Production by Perifused Ovine Placental Explants: Regulation by Growth Hormone-Releasing Hormone and Glucose
Placental Growth Hormone and Lactogen Production by Perifused Ovine Placental Explants: Regulation by Growth Hormone-Releasing Hormone and Glucose
复制标题
灌注羊胎盘外植体产生胎盘生长激素和泌乳素：生长激素释放激素和葡萄糖的调节
作者：
M. Lacroix;P. Bolifraud;D. Durieux;A. Pauloin;M. Vidaud;G. Kann
控制胎盘正常发育的因素知之甚少。我们以前曾报道存在绵羊胎盘生长激素（oPGH）和生长激素受体在绵羊胎盘，和oPGH生产的滋养外胚层和合胞体在怀孕的第二个月。为了确定调控oPGH产生的因素，我们开发了一种灌流系统，以测量第45天绵羊胎盘外植体的oPGH和绵羊胎盘催乳素（oPL）产生。两种激素的mRNA通过实时聚合酶链反应定量，在长达8小时的灌流期后收集的外植体中。绵羊PGH和oPL释放到培养基中的平均速率分别为2.45 ± 0.2和353.6 ± 13.6 ng/g/h。羊胎盘产生生长激素释放激素（GHRH），但除了GHRH的灌流培养基没有修改oPGH或oPL生产。在体内，oPGH的产生发生在妊娠的第30天和第60天之间。由于在此期间母体饮食的调节影响胎盘发育，因此评价了葡萄糖对oPGH和oPL产生的潜在调节。灌流培养基中补充葡萄糖导致4小时后oPGH释放呈浓度依赖性减少，但oPGH mRNA水平不受影响。oPL的产生不受葡萄糖的影响。因此，oPGH和oPL属于相同的生长激素/催乳素家族，但受葡萄糖的差异调节。在绵羊妊娠前三个月进行的代谢实验中应考虑绵羊PGH调节。
Abstract The factors controlling normal placental development are poorly understood. We have previously reported the presence of ovine placental growth hormone (oPGH) and growth hormone receptors in ovine placenta, and oPGH production by the trophectoderm and syncitium during the second month of pregnancy. To identify factors regulating oPGH production, we developed a perifusion system to measure oPGH and ovine placental lactogen (oPL) production by Day 45 ovine placental explants. The mRNAs for both hormones were quantitated by real-time polymerase chain reaction in explants collected after perifusion periods of up to 8 h. Ovine PGH and oPL were released into the medium at mean rates of 2.45 ± 0.2 and 353.6 ± 13.6 ng/g/h, respectively. Ovine placenta produces growth hormone-releasing hormone (GHRH), but addition of GHRH to the perifusion medium did not modify either oPGH or oPL production. In vivo, oPGH production occurs between Days 30 and 60 of pregnancy. Because modulation of the maternal diet during this period affects placental development, the potential regulation of oPGH and oPL production by glucose was evaluated. Glucose supplementation of the perifusion medium resulted in a concentration-dependent decrease in oPGH release after 4 h, but oPGH mRNA levels were not affected. Production of oPL was not affected by glucose. Thus, oPGH and oPL belong to the same growth hormone/prolactin family but are differentially regulated by glucose. Ovine PGH modulations should be taken into account in metabolic experiments performed during the first trimester of pregnancy in sheep.
培养的 3T3-F442A 脂肪细胞的慢性生长激素处理对两种葡萄糖转运蛋白的差异调节。
DOI：
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发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Tai,PK;Liao,JF;Chen,EH;Dietz,J;Schwartz,J;Carter-Su,C
通讯作者：
Carter-Su,C
生长激素信使核糖核酸在人体免疫系统中的定位——临床研究中心的研究。
DOI：
10.1210/jcem.81.3.8772612
发表时间：
1996
期刊：
The Journal of clinical endocrinology and metabolism.
影响因子：
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作者：
Wu,H;Devi,R;Malarkey,WB
通讯作者：
Malarkey,WB