Serum levels of soluble carbonic anhydrase IX are decreased in patients with diffuse cutaneous systemic sclerosis compared to those with limited cutaneous systemic sclerosis
Serum levels of soluble carbonic anhydrase IX are decreased in patients with diffuse cutaneous systemic sclerosis compared to those with limited cutaneous systemic sclerosis
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DOI：
10.5582/bst.2014.01020
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发表时间：
2014-01-01
影响因子：
5.5
通讯作者：
Ihn, Hironobu
中科院分区：
文献类型：
--
作者：
Makino, Katsunari;Jinnin, Masatoshi;Ihn, Hironobu
缺氧可能在系统性硬化症（SSc）的发病机制中起重要作用。碳酸酐酶IX（CA IX）是缺氧标志物之一，其胞外结构域可释放到血清中。然而，SSc中血清CA IX水平的临床意义仍然未知。本研究的目的是评估血清CA IX水平可以作为SSc的特异性疾病标志物的可能性。从SSc患者和健康对照获得血清样品。诊断为硬皮病谱系障碍（SSD）的患者，不符合ACR的SSc标准，但认为他们可能在未来发展SSc，也被纳入本研究。血清CA IX水平测定与特定的酶联免疫吸附试验。SSD患者的CA IX水平显著低于弥漫性皮肤SSc（dcSSc）、局限性皮肤SSc（lcSSc）和健康对照组。此外，我们发现dcSSc患者的值与lcSSc患者的值相比显著降低。血清CA IX水平可能有助于鉴别lcSSc和SSD。尽管SSc中存在缺氧，但血清CA IX水平降低可能表明对缺氧的反应受损，这导致持续缺氧状况。我们的结果表明，对缺氧的异常反应可能已经存在于SSD患者，并可能参与其发病机制。
Hypoxia may play an important role in the pathogenesis of systemic sclerosis (SSc). Carbonic anhydrase IX (CA IX) is one of the hypoxia markers and its extracellular domain can be released into the serum. However, the clinical significance of serum CA IX levels in SSc is still unknown. The aim of this study is to evaluate the possibility that serum CA IX levels can be a specific disease marker of SSc. Serum samples were obtained from SSc patients and healthy controls. Patients diagnosed as scleroderma spectrum disorder (SSD), who did not fulfill the ACR criteria of SSc but were thought that they might develop SSc in the future, were also included in this study. Serum CA IX levels were measured with specific enzyme-linked immunosorbent assays. SSD patients had significantly lower CA IX levels than diffuse cutaneous SSc (dcSSc), limited cuntaneous SSc (lcSSc) and healthy control groups. Also, we found a significant decrease in the values in dcSSc patients compared to those of lcSSc patients. Serum levels of CA IX may be useful for the differentiation of lcSSc from SSD. Decreased serum CA IX levels in spite of the presence of hypoxia in SSc may indicate an impaired response to hypoxia, which leads to the persistent hypoxic condition. Our results suggest that the abnormal response to hypoxia may already exist in SSD patients, and may be involved in its pathogenesis.