Water-soluble lipophilic MR contrast agents for cell membrane labeling.
Water-soluble lipophilic MR contrast agents for cell membrane labeling.
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DOI：
10.1007/s00775-015-1280-4
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发表时间：
2015-09
期刊：
影响因子：
--
通讯作者：
Meade TJ
中科院分区：
文献类型：
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作者：
Carney CE;MacRenaris KW;Meade TJ
磁共振成像的长期细胞跟踪需要造影剂的发展，这些造影剂既能标记细胞又能被细胞保留。一种有前景的长期细胞标记策略是开发锚定在细胞膜上的亲脂性Gd（III）基造影剂。我们以前报道过单体和多聚体亲脂剂的功效，并表明单体剂改善了细胞群的标记和对比度增强。在这里，我们报道了一系列具有不同烷基链组成的单体亲脂造影剂的合成、表征和体外测试。我们发现这些试剂在水中分散，在细胞膜上定位，并有效地标记HeLa和MCF7细胞。此外，与临床可用的ProHance®相比，这些药物在细胞中的保留率提高了10倍。
Long-term cell tracking with MR imaging necessitates the development of contrast agents that both label and are retained by cells. One promising strategy for long-term cell labeling is the development of lipophilic Gd(III)-based contrast agents that anchor into the cell membrane. We have previously reported the efficacy of monomeric and multimeric lipophilic agents and showed that the monomeric agents have improved labeling and contrast enhancement of cell populations. Here, we report on the synthesis, characterization, and in vitro testing of a series of monomeric lipophilic contrast agents with varied alkyl chain compositions. We show that these agents disperse in water, localize to the cell membrane, and label HeLa and MCF7 cells effectively. Additionally, these agents have up to 10-fold improved retention in cells compared to clinically available ProHance®.