Cooking with calcium: The recipes for composing global signals from elementary events
Cooking with calcium: The recipes for composing global signals from elementary events
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DOI：
10.1016/s0092-8674(00)80420-1
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发表时间：
1997-10-31
期刊：
影响因子：
64.5
通讯作者：
Lipp, P
中科院分区：
文献类型：
--
作者：
Bootman, MD;Berridge, MJ;Lipp, P
最近的研究表明，整体细胞内Ca2+信号来自亚细胞基本释放事件的总和和协调（例如，“Ca 2+脉冲”），尽管此类信号的募集模式尚不清楚。为了了解细胞如何利用基本的Ca2+释放事件，我们成像Ca2+瞬变诱发通过磷酸肌醇途径在HeLa细胞使用共聚焦显微镜。在导致全局Ca2+信号的起搏阶段，在（1）频率、（2）幅度和（3）空间域中募集基本Ca2+释放事件。由于每个数字基本事件有助于模拟胞质Ca 2+浓度的微小变化，因此三个域中的进展的净效应是将环境Ca 2+浓度推向信号变得再生的阈值，从而导致全局Ca 2+波。
Recent studies have suggested that global intracellular Ca2+ signals arise from the summation and coordination of subcellular elementary release events (e.g., ''Ca2+ puffs''), although the modes of recruitment of such signals are unknown. In order to understand how cells utilize elementary Ca2+ release events, we imaged Ca2+ transients evoked through the phosphoinositide pathway in HeLa cells using confocal microscopy. During the pacemaker phase leading to the global Ca2+ signal, elementary Ca2+ release events were recruited in (1) frequency, (2) amplitude, and (3) spatial domains. Since each digital elementary event contributes to a small change of the analog cytosolic Ca2+ concentration, the net effect of the advancement in the three domains is to drive the ambient Ca2+ concentration toward a threshold where the signal becomes regenerative, resulting in a global Ca2+ wave.