Alteration of cell wall composition leads to amphotericin B resistance in Aspergillus flavus
Alteration of cell wall composition leads to amphotericin B resistance in Aspergillus flavus
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DOI：
10.1111/j.1348-0421.1999.tb01231.x
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发表时间：
1999-01-01
影响因子：
2.6
通讯作者：
Ohnishi, Y
中科院分区：
文献类型：
--
作者：
Seo, K;Akiyoshi, H;Ohnishi, Y
通过将分生孢子连续转移到含有浓度逐步增加至100 μ g/ml的AmB的琼脂平板上，从AmB敏感的黄曲霉野生型菌株中分离出一种抗黄曲霉素B（AmB）突变株。菌丝体对多烯类抗生素AmB、制霉菌素和曲霉素具有特异性抗性，抗性菌丝体产生的原生质球对AmB的敏感性与野生型相同。细胞壁的化学分析表明，碱溶性和不溶性葡聚糖的水平显着较高的抗性菌丝体相比，在野生型。这些结果表明，菌丝体细胞壁成分的改变，特别是最外层的1，3-α-葡聚糖和蛋白复合物的改变，导致了A.黄的
An amphotericin B (AmB)-resistant mutant was isolated from a wild-type AmB-susceptible strain of Aspergillus flavus by serial transfer of conidia on agar plates containing stepwise increased concentrations of AmB up to 100 mu g ml(-1). The acquired resistance of mycelia was specific for polyene-antibiotics AmB, nystatin and trichomycin, Spheroplasts derived from the resistant mycelia were as susceptible to AmB as the wild-type. Chemical analysis of the cell wall revealed that levels of alkali-soluble and -insoluble glucans were significantly higher in the resistant mycelia as compared to those in the wild-type. When resistant mycelia were treated with SDS, they adsorbed as much AmB as wild-type mycelia, These results suggest that alterations in the cell wall components of mycelia, especially 1,3-alpha-glucan and protein complex in the outermost wall layer, lead to AmB resistance in A. flavus.