The leader sequence of tobacco mosaic virus RNA devoid of Watson-Crick secondary structure possesses a cooperatively melted, compact conformation
The leader sequence of tobacco mosaic virus RNA devoid of Watson-Crick secondary structure possesses a cooperatively melted, compact conformation
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DOI：
10.1016/j.bbrc.2007.04.152
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发表时间：
2007-06-22
影响因子：
3.1
通讯作者：
Spirin, Alexander S.
中科院分区：
文献类型：
--
作者：
Kovtun, Alexey A.;Shirokilch, Nikolay E.;Spirin, Alexander S.
烟草花叶病毒 (TMV) RNA 的 5'-非翻译区 (5'-UTR) 称为 omega 序列，被称为促进有效翻译起始的 mRNA 前导序列。该序列的中心部分由许多 CAA 重复序列组成，据报道这些重复序列主要负责增强 omega 前导序列的活性。在这项工作中，我们合成了含有天然 omega 序列或常规 (CAA)(n) 序列的多核糖核苷酸，并使用紫外分光光度法和分析超速离心方法对其进行了研究。结果表明，多核糖核苷酸表现出显着的低色度、加热时其结构的协同熔化、高熔化温度以及这种尺寸的紧密折叠RNA的典型沉降系数。因此，缺乏 Watson-Crick 型二级结构的 omega 前导序列及其核心 (CAA)(n) 重复序列似乎是 mRNA 结构良好的元件。 (c) 2007 Elsevier Inc. 保留所有权利。
The 5'-untranslated region (5'-UTR) of RNA of tobacco mosaic virus (TMV), called omega sequence, is known as an mRNA leader promoting efficient initiation of translation. The central part of the sequence consists of many CAA repeats, which were reported to be mainly responsible for the enhancing activity of the omega leader. In this work we synthesized the polyribonucleotides containing either the natural omega sequence or the regular (CAA)(n) sequence, and studied them using UV spectrophotometry and analytical ultracentrifugation methods. It was demonstrated that the polyribonucleotides manifest significant hypochromicity, cooperative melting of their structures upon heating, high melting temperature, and the sedimentation coefficients typical of compactly folded RNAs of this size. Thus, the omega leader and its core (CAA)(n) repeat sequence devoid of secondary structure of the Watson-Crick type seem to be well structured elements of mRNA. (c) 2007 Elsevier Inc. All rights reserved.