Nucleotide sequence of the gene encoding Clostridium botulinum neurotoxin type D.
Nucleotide sequence of the gene encoding Clostridium botulinum neurotoxin type D.
复制标题
编码肉毒梭菌神经毒素 D 型的基因的核苷酸序列。
作者：
T. Binz;Hisao Kurazono;M. Popoff;Melvin W. Eklund;Genji Sakaguchi;Shunji Kozaki;Kerstin Krieglstein;Agnes Henschen;D. M. Gill;H. Niemann
七种血清学上不同类型的肉毒梭菌神经毒素（命名为BoNT/A至BoNT/G）被合成为约150 kDa的单链多肽。蛋白水解活化产生通过单个二硫键连接的N-末端轻链（=50 kDa）和C-末端重链（= 100 kDa）。BoNT/D与BoNT/Cl一样（1），由噬菌体编码（2，3）。以下所示的序列是由克隆的噬菌体片段建立的，其大小小于2.0kb（实验由ZKBS Az 1494/1注册）。用合成的23-mer寡核苷酸鉴定初始克隆，所述寡核苷酸在摆动位置仅含有A或T残基。该序列（下划线）是从通过溴化氰片段化从纯化的BoNT/D获得的肽推导出来的。的序列
The seven serologically distinct types of Clostridium botulinum neurotoxins, designated BoNT/A to BoNT/G, are synthesized as single-chain polypeptides of about 150 kDa. Proteolytic activation yields N-terminal light (=50 kDa) and C-terminal heavy chains (= 100 kDa) connected by single disulfide bonds. BoNT/D, like BoNT/Cl (1), is encoded by a bacteriophage (2, 3). The sequence shown below was established from cloned bacteriophage fragments the sizes of which were smaller than 2.0 kb (experiments registered by ZKBS Az 1494/1). The initial clone was identified with a synthetic 23-mer oligonucleotide containing only A or T residues in the wobble positions. The sequence (underlined) was deduced from a peptide obtained from purified BoNT/D by cyanogen bromide fragmentation. The sequence of