Artificial Antigen-Presenting Cell Fabrication for Murine T Cell Expansion.
Artificial Antigen-Presenting Cell Fabrication for Murine T Cell Expansion.
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用于鼠 T 细胞扩增的人工抗原呈递细胞制造。
DOI：
10.1002/cpz1.976
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Schneck,JonathanP
中科院分区：
文献类型：
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作者：
Omotoso,MaryO;Lanis,MaraR;Schneck,JonathanP
抗原呈递细胞（APC），如树突状细胞和巨噬细胞，具有独特的能力，通过负载肽的主要组织相容性复合物（信号1）来调查身体并向T细胞呈递信息。这种呈递，沿着共刺激信号（信号2），导致T细胞的活化和随后的扩增。这个过程可以被利用和用于治疗应用，但患者来源的APC的使用可能是复杂和低效的。或者，人工APC（aAPC）提供了一种简化的方法，通过呈递两种必要的刺激信号来实现T细胞活化。该方案描述了利用磁性纳米颗粒和刺激蛋白来创建aAPC，可用于激活和扩增抗原特异性T细胞，用于基础和翻译免疫学和免疫治疗研究。© 2024 Wiley Periodicals LLC.基本方案1：用于aAPC制造的蛋白质和颗粒修饰基本方案2：通过缀合蛋白的免疫标记进行aAPC验证支持方案1：aAPC储备浓度的定量基本方案3：用于鼠CD8+T细胞活化的aAPC使用的测定支持方案2：鼠CD8+T细胞的分离
Antigen‐presenting cells (APCs), such as dendritic cells and macrophages, have a unique ability to survey the body and present information to T cells via peptide‐loaded major histocompatibility complexes (signal 1). This presentation, along with a co‐stimulatory signal (signal 2), leads to activation and subsequent expansion of T cells. This process can be harnessed and utilized for therapeutic applications, but the use of patient‐derived APCs can be complex and inefficient. Alternatively, artificial APCs (aAPCs) provide a simplified method to achieve T cell activation by presenting the two necessary stimulatory signals. This protocol describes the utilization of magnetic nanoparticles and stimulatory proteins to create aAPCs that can be employed for activating and expanding antigen‐specific T cells for both basic and translational immunology and immunotherapy studies. © 2024 Wiley Periodicals LLC.Basic Protocol 1: Protein and particle modification for aAPC fabricationBasic Protocol 2: aAPC validation by immunolabeling of conjugated proteinSupport Protocol 1: Quantification of aAPC stock concentrationBasic Protocol 3: Determination of aAPC usage for murine CD8+T cell activationSupport Protocol 2: Isolation of murine CD8+T cells