Metastable hybridization-based DNA information storage to allow rapid and permanent erasure.
Metastable hybridization-based DNA information storage to allow rapid and permanent erasure.
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DOI：
10.1038/s41467-020-18842-6
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发表时间：
2020-10-06
影响因子：
16.6
通讯作者：
Zhang DY
中科院分区：
文献类型：
--
作者：
Kim J;Bae JH;Baym M;Zhang DY
由于DNA合成和测序的进步，DNA作为信息存储介质的潜力正在迅速增长。然而，DNA的化学稳定性对DNA序列中编码的信息的完全擦除提出了挑战。在这里，我们将信息编码在DNA信息解决方案中，这是一种真消息编码和错误消息编码的寡核苷酸的混合物，并使信息能够快速和永久地擦除。真实的信息通过与“真理标记”寡核苷酸的杂交来区分，只有真实的信息才能被阅读；真理标记的结合可以有效地随机化，即使在短暂的高温下也是如此。我们展示了8个独立的位图图像在25 °C下存储65天后可以稳定地编码和读取，平均正确信息召回率超过99%，这意味着在25 °C下的半衰期超过15年，然而，加热到95 °C 5分钟会永久删除消息。DNA的化学稳定性使得完全删除DNA编码的数据变得困难。在这里，作者混合了真和假的信息，通过是否绑定了真理标记寡头来区分它，并表明短暂的高温暴露会随机化阻止数据恢复的真理标记的绑定。
The potential of DNA as an information storage medium is rapidly growing due to advances in DNA synthesis and sequencing. However, the chemical stability of DNA challenges the complete erasure of information encoded in DNA sequences. Here, we encode information in a DNA information solution, a mixture of true message- and false message-encoded oligonucleotides, and enables rapid and permanent erasure of information. True messages are differentiated by their hybridization to a "truth marker” oligonucleotide, and only true messages can be read; binding of the truth marker can be effectively randomized even with a brief exposure to the elevated temperature. We show 8 separate bitmap images can be stably encoded and read after storage at 25 °C for 65 days with an average of over 99% correct information recall, which extrapolates to a half-life of over 15 years at 25 °C. Heating to 95 °C for 5 minutes, however, permanently erases the message. The chemical stability of DNA makes complete erasure of DNA-encoded data difficult. Here the authors mix true and false messages, differentiated by whether a truth marker oligo is bound to it, and show that brief exposure to elevated temperatures randomizes the binding of truth markers preventing data recovery.